Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1973-4-2
pubmed:abstractText
The binding of antigen to cells with antibody on their surface has been studied in a model system consisting of murine myeloma cells (MOPC 315) and DNP conjugates. Specific binding occurred between the DNP groups of DNP conjugates and cell surface immunoglobulin. Using this model, the binding affinities of multivalent and univalent DNP conjugates were measured directly by equilibrium-binding techniques and indirectly by displacement of bound conjugate with univalent hapten. With both approaches the multivalent conjugate was shown to bind to cells with an avidity 100-300 fold greater than the univalent hapten. Nonspecific binding of unrelated protein and repeated washing of cells was found to markedly dedecrease the specific binding of univalent conjugates, presumably because the relatively weak bonds dissociate readily.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-14030666, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-14279047, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-14319411, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-4104294, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-4108348, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-4110985, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-4113719, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-4117184, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-4117541, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-4160399, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-4169723, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-4177521, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-4185494, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-4554743, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-4556115, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-4900673, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-4928749, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-4941003, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-4942636, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-4981687, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-5037346, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-5061978, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-5166611, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-5347697, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-5495449, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-5511573, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-5553718, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-5926449, http://linkedlifedata.com/resource/pubmed/commentcorrection/4734402-5965154
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
137
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
301-16
pubmed:dateRevised
2010-6-22
pubmed:meshHeading
pubmed:year
1973
pubmed:articleTitle
Binding of antigen by immunocytes. I. Effect of ligand valence on binding affinity of MOPC 315 cells for DNP conjugates.
pubmed:publicationType
Journal Article