|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
1973-5-18
|
|
pubmed:abstractText |
Membranoproliferative nephritis in children is frequently associated with a hypocomplementemia produced at least in part by C3 breakdown mediated by a circulating anticomplementary factor known as C3 nephritic factor (C3NeF). C3 breakdown by this factor in vitro requires the presence of a pseudoglobulin cofactor and magnesium. The present study provides evidence that properdin factor B (C3 proactivator) is activated in the nephritic factor reaction and is the direct mediator of C3 breakdown by C3NeF. Depletion of factor B from mixtures of normal human serum (NHS) and plasma from a patient with membranoproliferative nephritis (MPP), either by heating or by immune equivalence absorption, blocks C3 breakdown by C3NeF. Addition of purified factor B to these mixtures restores the anticomplementary effect. When purified factor B is added to mixtures of MPP and purified C3, breakdown also occurs. Associated with the C3 breakdown is a change in the electrophoretic mobility of factor B from the beta to the gamma position, a shift which has been associated with cleavage activation of the molecule. Further, serum factor B levels are often low in patients with membranoproliferative nephritis and bear a rough inverse correlation with C3NeF levels. It thus appears that factor B is the previously described heat-labile C3NeF cofactor. Whether the C3NeF reaction proceeds via a pathway comparable to that activated by the cobra venom factor or via that activated by zymosan or inulin cannot be determined from the present data.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-14201535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-14316946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-14324514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-4097129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-4101070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-4105938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-4106150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-4111773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-4164258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-4166187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-4171046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-4176223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-4180576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-4192571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-4625395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-4671224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-4956917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-4975569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-5026036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-5093780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-5101779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-5120401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-5349693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-5499437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4693654-6074005
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0021-9738
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
52
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
896-904
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:4693654-Absorption,
pubmed-meshheading:4693654-Blood Protein Electrophoresis,
pubmed-meshheading:4693654-Complement System Proteins,
pubmed-meshheading:4693654-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:4693654-Hot Temperature,
pubmed-meshheading:4693654-Humans,
pubmed-meshheading:4693654-Immunoelectrophoresis,
pubmed-meshheading:4693654-Nephritis,
pubmed-meshheading:4693654-Properdin,
pubmed-meshheading:4693654-Zymosan
|
|
pubmed:year |
1973
|
|
pubmed:articleTitle |
Hypocomplementemia of membranoproliferative nephritis. Dependence of the nephritic factor reaction on properdin factor B.
|
|
pubmed:publicationType |
Journal Article
|
