|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
5
|
|
pubmed:dateCreated |
1973-5-18
|
|
pubmed:abstractText |
1. Analysis of transport of d-galactose was complicated by metabolism of the compound but appeared to have two components: a substrate-saturable component and a diffusion component. At low substrate concentration (<1mm) active transport was observed. Accumulation of galactose was largely independent of Na(+) concentration. The apparent K(m) for this component was 0.2mm. At substrate concentrations above 1mm the active transport system appeared saturated and further increases in substrate concentration resulted in a linear increase in the rate of galactose accumulation, but no concentration gradient was formed. 2. d-[1-(14)C]Galactose (2mm) was metabolized to (14)CO(2) by rat kidney-cortex slices incubated at 37 degrees C, at the rate of 68nmol/h per 100mg of tissue. 3. Intracellular components from such incubations were separated into a neutral fraction, the only major labelled component being galactose, and a phosphorylated fraction. 4. Phosphorylated metabolites found in galactose-incubated slices increased with increasing substrate concentration and achieved a limiting value of 0.42mm after 60min of incubation. 5. Galactose uptake was inhibited by anaerobiosis, dinitrophenol and phlorrhizin. 6. Methyl alpha-d-glucoside and d-glucose partially inhibited galactose uptake only at ratios of 100:1. 7. The presence of pyruvate did not decrease galactose metabolism although it did decrease production of (14)CO(2) from [1-(14)C]galactose. Gluconeogenesis occurred in the presence of pyruvate and (14)C from galactose was found in glucose. 8. Rat kidney-cortex slices metabolized 2mm-[1-(14)C]galactonate to (14)CO(2) at a rate of 20nmol/h per 100mg of tissue.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-13062066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-13610908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-13630880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-13743394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-14249137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-14304841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-14493965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-14493967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-4304332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-5160733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-5459999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-5475338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-5491216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-5573951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-5645754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-5697387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-5912020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-5920808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-5938779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-6031498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-6032461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-6049926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-6049927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4656597-6052957
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0264-6021
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
129
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1109-18
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:4656597-Anaerobiosis,
pubmed-meshheading:4656597-Animals,
pubmed-meshheading:4656597-Biological Transport, Active,
pubmed-meshheading:4656597-Carbon Dioxide,
pubmed-meshheading:4656597-Carbon Isotopes,
pubmed-meshheading:4656597-Chromatography, DEAE-Cellulose,
pubmed-meshheading:4656597-Chromatography, Gas,
pubmed-meshheading:4656597-Dinitrophenols,
pubmed-meshheading:4656597-Galactose,
pubmed-meshheading:4656597-Glucose,
pubmed-meshheading:4656597-Glycosides,
pubmed-meshheading:4656597-Hexoses,
pubmed-meshheading:4656597-Kidney,
pubmed-meshheading:4656597-Kidney Cortex,
pubmed-meshheading:4656597-Male,
pubmed-meshheading:4656597-Phlorhizin,
pubmed-meshheading:4656597-Pyruvates,
pubmed-meshheading:4656597-Rats,
pubmed-meshheading:4656597-Sodium
|
|
pubmed:year |
1972
|
|
pubmed:articleTitle |
Transport and metabolism of galactose in rat kidney cortex.
|
|
pubmed:publicationType |
Journal Article,
In Vitro
|
