Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
28
|
pubmed:dateCreated |
1972-4-24
|
pubmed:abstractText |
The fluorescent dye 1,8-anilinonaphthalenesulfonate competed with thyroxine for binding to thyroxine-binding globulin. Fluorescence analysis indicated that the dye bound to the globulin in a molar ratio of 1:1 and with an association constant (at 23 degrees C) of 4.19 x10(6)M(-1), and that thyroxine bound to the globulin in a molar ratio of 1:1 and with an association constant (at 23 degrees C) of 2.35x10(10)M(-1). Displacement of globulin-bound dye by thyroxine was shown by fluorescence quenching, and displacement of globulin-bound thyroxine by dye was demonstrated by ultrafiltration.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
|
pubmed:issn |
0036-8075
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
24
|
pubmed:volume |
175
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1378-80
|
pubmed:dateRevised |
2007-8-17
|
pubmed:meshHeading |
pubmed-meshheading:4621782-Binding Sites,
pubmed-meshheading:4621782-Energy Transfer,
pubmed-meshheading:4621782-Filtration,
pubmed-meshheading:4621782-Fluorescent Dyes,
pubmed-meshheading:4621782-Molecular Weight,
pubmed-meshheading:4621782-Optical Rotatory Dispersion,
pubmed-meshheading:4621782-Protein Binding,
pubmed-meshheading:4621782-Spectrum Analysis,
pubmed-meshheading:4621782-Temperature,
pubmed-meshheading:4621782-Thyroxine,
pubmed-meshheading:4621782-Thyroxine-Binding Proteins
|
pubmed:year |
1972
|
pubmed:articleTitle |
Thyroxine-binding globulin: characterization of the binding site with a fluorescent dye as a probe.
|
pubmed:publicationType |
Journal Article
|