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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1974-7-16
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pubmed:abstractText |
A procedure for the preparative isolation of Escherichia coli cell wall, membrane, and deoxyribonucleic acid (DNA)-envelope complex fragments has been developed. The envelope fragments were produced by controlled mechanical cell breakage and isolated by density gradient centrifugation and subsequent preparative free-flow electrophoresis. The DNA-envelope complex fragments were shown to contain biochemical markers of both the cell wall and the membrane and by electron microscopy to be cell envelope fragments containing wall/membrane adhesion zones.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597456-13862582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597456-4099097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597456-4159978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597456-4181162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597456-4186529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597456-4550815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597456-4555955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597456-4570960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597456-4868245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597456-4911849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597456-4923077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597456-4941569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597456-4977759
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Polysaccharides, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Succinate Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
118
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
646-53
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:4597456-Amino Acids,
pubmed-meshheading:4597456-Bacterial Proteins,
pubmed-meshheading:4597456-Binding Sites,
pubmed-meshheading:4597456-Carbon Radioisotopes,
pubmed-meshheading:4597456-Cell Fractionation,
pubmed-meshheading:4597456-Cell Membrane,
pubmed-meshheading:4597456-Cell Wall,
pubmed-meshheading:4597456-Centrifugation, Density Gradient,
pubmed-meshheading:4597456-DNA, Bacterial,
pubmed-meshheading:4597456-Electrophoresis,
pubmed-meshheading:4597456-Escherichia coli,
pubmed-meshheading:4597456-Lipopolysaccharides,
pubmed-meshheading:4597456-Methods,
pubmed-meshheading:4597456-Microscopy, Electron,
pubmed-meshheading:4597456-Polysaccharides, Bacterial,
pubmed-meshheading:4597456-Succinate Dehydrogenase,
pubmed-meshheading:4597456-Thymidine,
pubmed-meshheading:4597456-Tritium
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pubmed:year |
1974
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pubmed:articleTitle |
Deoxyribonucleic acid-envelope complexes isolated from Escherichia coli by free-flow electrophoresis: biochemical and electron microscope characterization.
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pubmed:publicationType |
Journal Article
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