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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1974-7-16
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pubmed:abstractText |
The limiting sizes of molecules that can permeate the intact cell wall and protoplast membrane of Saccharomyces cerevisiae were determined from the inflection points in a triphasic pattern of passive equilibrium uptake values obtained with a series of inert probing molecules varying in molecular size. In the phase identified with the yeast protoplast, the uptake-exclusion threshold corresponded to a monodisperse ethylene glycol of molecular weight = 110 and Einstein-Stokes hydrodynamic radius (r(ES)) = 0.42 nm. In the cell wall phase, the threshold corresponded to a polydisperse polyethylene glycol of number-average molecular weight ( M(n)) = 620 and average radius (r(ES)) = 0.81 nm. The third phase corresponded to complete exclusion of larger molecules. The assessment of cell wall porosity was confirmed by use of a second method involving analytical gel chromatographic analyses of the molecular weight distribution for a single polydisperse polyglycol before and after uptake by the cells, which indicated a quasi-monodisperse threshold for the cell wall of M(n) = 760 and r(ES) = 0.89 nm. The results were reconciled with two situations in which much larger protein molecules previously have been reported able to penetrate the yeast cell wall.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597447-13534699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597447-13869665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597447-13897940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597447-14137635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597447-14236277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597447-14255711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597447-14333566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597447-14800892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597447-14944578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597447-4294420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597447-4565542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597447-4999413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597447-5001873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597447-5300486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597447-5344109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597447-5488470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597447-5926738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4597447-6044286
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
118
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
534-40
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:4597447-Biological Transport,
pubmed-meshheading:4597447-Carbon Radioisotopes,
pubmed-meshheading:4597447-Cell Membrane,
pubmed-meshheading:4597447-Cell Membrane Permeability,
pubmed-meshheading:4597447-Cell Wall,
pubmed-meshheading:4597447-Chromatography,
pubmed-meshheading:4597447-Dextrans,
pubmed-meshheading:4597447-Diffusion,
pubmed-meshheading:4597447-Glycols,
pubmed-meshheading:4597447-Inulin,
pubmed-meshheading:4597447-Methods,
pubmed-meshheading:4597447-Molecular Weight,
pubmed-meshheading:4597447-Protoplasts,
pubmed-meshheading:4597447-Saccharomyces cerevisiae
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pubmed:year |
1974
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pubmed:articleTitle |
Porosity of the yeast cell wall and membrane.
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pubmed:publicationType |
Journal Article
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