Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1973-12-16
pubmed:abstractText
Escherichia coli fil ts forms multinucleate filaments when suspensions of about 10(7) organisms per ml are shifted from 37 to 43 C in rich medium. Occasional septation continues, chiefly at the poles, and immediately becomes more frequent when the filaments are returned to 37 C. The addition of chloramphenicol (200 mug/ml) at either temperature initially stimulates the formation of polar septa. When very dilute suspensions of the strain (<10(6) organisms per ml) are shifted to the restrictive temperature, the inhibition of septation is more complete and only seldom reversible. Conversely, cell division is little affected when suspensions of >10(8) organisms per ml, or microcolonies of several hundred organisms on agar, are incubated at 43 C; evidence is presented that this is a consequence of a slight reduction in the mutant's growth rate. In certain media, septation is blocked irreversibly by even brief exposure to 43 C, after which cell elongation without division proceeds at 37 C for some hours. Several findings, when considered together, suggest that the cytoplasmic membrane is normal at the restrictive temperature, and that the block in septation is caused by a defect in the cell wall: it is largely overcome by NaCl, but not by sucrose; in some circumstances the filaments become swollen and develop localized bulges in the wall, yet the membrane remains intact and retains its selective permeability; lastly, the strain is insensitive to deoxycholate at both temperatures. The mutation has been mapped between arg B and thr, at a locus which appears to be distinct from others known primarily to influence cell division.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-16559077, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4401416, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4555247, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4557275, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4558189, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4558190, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4560759, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4567159, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4862197, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4866336, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4886279, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4892005, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4896021, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4907727, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4908787, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4918631, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4924005, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4929323, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4933802, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4939758, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4941947, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4950294, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4966824, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-4977057, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-5324449, http://linkedlifedata.com/resource/pubmed/commentcorrection/4583249-5543950
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
116
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
741-50
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1973
pubmed:articleTitle
Regulation of cell division in a temperature-sensitive division mutant of Escherichia coli.
pubmed:publicationType
Journal Article