Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1973-1-18
pubmed:abstractText
In Escherichia coli K-12 the proteins involved in the dissimilation of mannitol and sorbitol are specified by two separate gene clusters. The mannitol cluster appears to consist of a regulatory gene mtlC, a gene mtlA coding an enzyme II complex of the phosphoenolpyruvate phosphotransferase system, and a gene mtlD coding a mannitol-1-phosphate dehydrogenase. Three corresponding genes, sblC, sblA, and sblD, exist for the sorbitol pathway. In both pathways the hexitol captured from the medium and delivered into the cytoplasm as a phosphorylated compound is dehydrogenated to fructose-6-phosphate. The enzyme II complex for sorbitol is able to catalyze the phosphorylation also of mannitol if this substrate is present at high concentrations. Consequently mtlA(-) mutants lacking the enzyme II complex for mannitol can grow on mannitol either if the sorbitol phosphorylating system is preinduced by sorbitol or if mtlA is suppressed by a mutation of sblC to constitutivity. In wild-type cells, the induction of the enzymes in the mannitol pathway and dissimilation of the substrate are not prevented by glucose. The sorbitol system, however, is sensitive to glucose and to mannitol as well. In the suppressed strains (mtlA(-), sblC(c)) in which mannitol is utilized through the sorbitol enzyme, glucose becomes effective in restraining the consumption of mannitol, causing a definite diauxie. Moreover, in a mixture of mannitol and sorbitol, the latter is utilized preferentially. This reversal of normal diauxic pattern is consequent to the fact that the enzyme II complex for sorbitol has relatively poor affinity for mannitol.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/4563979-13331868, http://linkedlifedata.com/resource/pubmed/commentcorrection/4563979-13762204, http://linkedlifedata.com/resource/pubmed/commentcorrection/4563979-13794037, http://linkedlifedata.com/resource/pubmed/commentcorrection/4563979-14103281, http://linkedlifedata.com/resource/pubmed/commentcorrection/4563979-14154462, http://linkedlifedata.com/resource/pubmed/commentcorrection/4563979-14231443, http://linkedlifedata.com/resource/pubmed/commentcorrection/4563979-14392137, http://linkedlifedata.com/resource/pubmed/commentcorrection/4563979-14468226, http://linkedlifedata.com/resource/pubmed/commentcorrection/4563979-4289962, http://linkedlifedata.com/resource/pubmed/commentcorrection/4563979-4559737, http://linkedlifedata.com/resource/pubmed/commentcorrection/4563979-4860714, http://linkedlifedata.com/resource/pubmed/commentcorrection/4563979-4863695, http://linkedlifedata.com/resource/pubmed/commentcorrection/4563979-4914079, http://linkedlifedata.com/resource/pubmed/commentcorrection/4563979-4918631, http://linkedlifedata.com/resource/pubmed/commentcorrection/4563979-4989971, http://linkedlifedata.com/resource/pubmed/commentcorrection/4563979-4996112, http://linkedlifedata.com/resource/pubmed/commentcorrection/4563979-5332079, http://linkedlifedata.com/resource/pubmed/commentcorrection/4563979-5541002, http://linkedlifedata.com/resource/pubmed/commentcorrection/4563979-5674056
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
112
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
840-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1972
pubmed:articleTitle
Reversal of the mannitol-sorbitol diauxie in Escherichia coli.
pubmed:publicationType
Journal Article