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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1972-8-3
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pubmed:abstractText |
The level of messenger ribonucleic acid specific for the argECBH gene cluster (arg-mRNA) of Escherichia coli was measured by deoxyribonucleic acid-ribonucleic acid hybridization in a number of strains. During the first 10 min after removal of arginine (derepression), the rate of arg-mRNA accumulation was six to ten times greater than that found in arginine-repressed argR(+) cells. In the absence of arginine, l-canavanine (200 mug/ml) repressed arg-mRNA synthesis to a level only 20 to 30% lower than that found after arginine deprivation. High levels of arg-mRNA were produced by argR(-) strains with or without added arginine. Within about 2 min after arginine addition to argR(+) cells, the rate of synthesis of arg-mRNA reached the repressed level. Likewise, 2.5 min after rifampin addition, all transcription of arg-mRNA was completed. These data are consistent with the view that arginine signals repression by inhibiting the initiation of transcription of arg-mRNA mediated in some way by the argR gene. The kinetics of arg-mRNA accumulation and the kinetics of completion of transcription together with the profile of hybridizable arg-mRNA in sucrose density gradients (major component 16S) suggest that the argECBH gene cluster is transcribed in short pieces rather than as a single unit.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-13670913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-13750787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-13900532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-13939775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-14168689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-14168690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-14265747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-14292146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-14467046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-14467697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-15436457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-4324343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-4860986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-4877125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-4877268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-4905438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-4919771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-4922206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-4923118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-4926363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-4927673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-4938817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-4938818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-4939044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-4939045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-4941555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-4946188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-4955314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-4986890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-4992222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-5001420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-5323612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-5323613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-5361395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4555419-5542011
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arginine,
http://linkedlifedata.com/resource/pubmed/chemical/Canavanine,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Ornithine Carbamoyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Rifampin,
http://linkedlifedata.com/resource/pubmed/chemical/Sucrose,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium,
http://linkedlifedata.com/resource/pubmed/chemical/Uridine
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
110
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
945-54
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:4555419-Arginine,
pubmed-meshheading:4555419-Canavanine,
pubmed-meshheading:4555419-Centrifugation, Density Gradient,
pubmed-meshheading:4555419-DNA, Bacterial,
pubmed-meshheading:4555419-Escherichia coli,
pubmed-meshheading:4555419-Genes,
pubmed-meshheading:4555419-Genetic Code,
pubmed-meshheading:4555419-Genetics, Microbial,
pubmed-meshheading:4555419-Mutation,
pubmed-meshheading:4555419-Nucleic Acid Hybridization,
pubmed-meshheading:4555419-Ornithine Carbamoyltransferase,
pubmed-meshheading:4555419-RNA, Bacterial,
pubmed-meshheading:4555419-RNA, Messenger,
pubmed-meshheading:4555419-Rifampin,
pubmed-meshheading:4555419-Sucrose,
pubmed-meshheading:4555419-Transduction, Genetic,
pubmed-meshheading:4555419-Tritium,
pubmed-meshheading:4555419-Uridine
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pubmed:year |
1972
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pubmed:articleTitle |
Arginine control of transcription of argECBH messenger ribonucleic acid in Escherichia coli.
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pubmed:publicationType |
Journal Article
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