|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1972-6-20
|
|
pubmed:abstractText |
Three ubiquinone-deficient mutants of Escherichia coli unable to convert 4-hydroxybenzoate into 3-octaprenyl-4-hydroxybenzoate were isolated and examined. The results of genetic analysis suggest that each of the mutants carries a mutation in a gene designated ubiA which can be represented at minute 79 on the E. coli chromosome map. The conversion of 4-hydroxybenzoate into 3-octaprenyl-4-hydroxybenzoate, catalyzed by 4-hydroxybenzoate octaprenyltransferase, was studied with a strain of E. coli that is blocked in the common pathway of aromatic biosynthesis and consequently accumulates the precursor of the side chain of ubiquinone. Both the side-chain precursor and 4-hydroxybenzoate octaprenyltransferase were shown to be membrane-bound. The enzyme required Mg(2+) for optimal activity. The ubiA(-) mutants were found to lack 4-hydroxybenozate octaprenyltransferase activity, which suggested that the ubiA gene is the structural gene coding for this enzyme.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4552989-14171457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4552989-14221874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4552989-14273645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4552989-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4552989-4292725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4552989-4305154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4552989-4311025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4552989-4323297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4552989-4330424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4552989-4333375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4552989-4870277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4552989-4897112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4552989-4918631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4552989-4942305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4552989-5326114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4552989-5332865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4552989-5834234
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0021-9193
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
110
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
18-25
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:4552989-Benzoates,
pubmed-meshheading:4552989-Carbon Isotopes,
pubmed-meshheading:4552989-Chromatography, Thin Layer,
pubmed-meshheading:4552989-Chromosome Mapping,
pubmed-meshheading:4552989-Coliphages,
pubmed-meshheading:4552989-Enzyme Activation,
pubmed-meshheading:4552989-Escherichia coli,
pubmed-meshheading:4552989-Genes,
pubmed-meshheading:4552989-Genetics, Microbial,
pubmed-meshheading:4552989-Kinetics,
pubmed-meshheading:4552989-Magnesium,
pubmed-meshheading:4552989-Mutation,
pubmed-meshheading:4552989-Subcellular Fractions,
pubmed-meshheading:4552989-Terpenes,
pubmed-meshheading:4552989-Transduction, Genetic,
pubmed-meshheading:4552989-Transferases,
pubmed-meshheading:4552989-Ubiquinone
|
|
pubmed:year |
1972
|
|
pubmed:articleTitle |
Biochemical and genetic studies on ubiquinone biosynthesis in Escherichia coli K-12:4-hydroxybenzoate octaprenyltransferase.
|
|
pubmed:publicationType |
Journal Article
|
