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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1981-3-17
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pubmed:abstractText |
Available cultures of Thiobacillus ferrooxidans were found to be contaminated with bacteria very similar to Thiobacillus acidophilus. The experiments described were performed with a homogeneous culture of Thiobacillus ferrooxidans. Pyrite (FeS2) was oxidized by Thiobacillus ferrooxidans grown on iron (Fe2+), elemental sulphur (S0) or FeS2. Evidence for the direct utilization of the sulphur moiety of pyrite by Thiobacillus ferrooxidans was derived from the following observations: a. Known inhibitors of Fe2+ and S0 oxidation, NaN3 and NEM, respectively, partially abolished FeS2 oxidation. b. A b-type cytochrome was detectable in FeS2- and S0-grown cells but not in Fe2+-grown cells. c. FeS2 and S0 reduced b-type cytochromes in whole cells grown on S0. d. CO2 fixation at pH 4.0 per mole of oxygen consumed was the highest with S0, lowest with Fe2+ and medium with FeS2 as substrate. e. Bacterial Fe2+ oxidation was found to be negligible at pH 5.0 whereas both FeS2 and S0 oxidation was still appreciable above this pH. f. Separation of pyrite and bacteria by means of a dialysis bag caused a pronounced drop of the oxidation rate which was similar to the reduction of pyrite oxidation by NEM; indirect oxidation of the sulphur moiety by Fe3+ was not affected by separation of pyrite and bacteria. Bacterial oxidation and utilization of the sulphur moiety of pyrite were relatively more important with increasing pH.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Azides,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Dioxide,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochromes,
http://linkedlifedata.com/resource/pubmed/chemical/Ethylmaleimide,
http://linkedlifedata.com/resource/pubmed/chemical/Ferrous Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Iron,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfides,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfur
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pubmed:status |
MEDLINE
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pubmed:issn |
0003-6072
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
423-35
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pubmed:dateRevised |
2000-12-18
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pubmed:meshHeading |
pubmed-meshheading:45294-Azides,
pubmed-meshheading:45294-Carbon Dioxide,
pubmed-meshheading:45294-Cytochromes,
pubmed-meshheading:45294-Energy Metabolism,
pubmed-meshheading:45294-Ethylmaleimide,
pubmed-meshheading:45294-Ferrous Compounds,
pubmed-meshheading:45294-Hydrogen-Ion Concentration,
pubmed-meshheading:45294-Iron,
pubmed-meshheading:45294-Sulfides,
pubmed-meshheading:45294-Sulfur,
pubmed-meshheading:45294-Thiobacillus
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pubmed:year |
1979
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pubmed:articleTitle |
Pyrite oxidation by Thiobacillus ferrooxidans with special reference to the sulphur moiety of the mineral.
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pubmed:publicationType |
Journal Article
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