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rdf:type |
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lifeskim:mentions |
umls-concept:C0011529,
umls-concept:C0018321,
umls-concept:C0028580,
umls-concept:C0033727,
umls-concept:C0037633,
umls-concept:C0040087,
umls-concept:C0521116,
umls-concept:C0599956,
umls-concept:C1260969,
umls-concept:C1261322,
umls-concept:C1382100,
umls-concept:C1441506
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pubmed:issue |
5
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pubmed:dateCreated |
1974-8-23
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pubmed:abstractText |
Methods are outlined for assigning the guanine-N(1)H and thymine-N(3)H protons to particular base pairs in the proton nuclear magnetic resonance spectra of double-stranded oligodeoxyribonucleotides of known sequence in aqueous solution. Ring current calculations have been used to evaluate the upfield shifts of the guanine-N(1)H and thymine-N(3)H protons from the pyrimidine and purine rings of nearest-neighbor base pairs in DNA B-type double-helical structures. Chemical shifts of 13.6 +/- 0.1 ppm and 14.6 +/- 0.2 ppm are assigned to the guanine-N(1)H proton of an isolated G.C base pair and the thymine-N(3)H proton of an isolated A.T base pair, respectively.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
71
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1945-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1974
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pubmed:articleTitle |
Proton nuclear magnetic resonance investigations and ring current calculations of guanine N-1 and thymine N-3 hydrogen-bonded protons in double-helical deoxyribonucleotides in aqueous solution.
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pubmed:publicationType |
Journal Article
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