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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1973-8-10
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pubmed:abstractText |
Concanavalin A (Con A) has been used to rapidly and selectively agglutinate murine and avian oncornavirions from culture medium or plasma. The agglutinated virus was concentrated rapidly and gently by low-speed centrifugation and solubilization with alpha-methyl mannoside. Infectious virus was purified 2.3 times with respect to nucleic-acid content, and more than 60% of its infectivity was recovered. Infectious particles of densities 1.18 and 1.16 g/cm(3) were found in mouse cells infected with Friend virus. Con A reacted only with particles of density 1.16 g/cm(3), indicating heterogeneity with respect to carbohydrate content or structure as well as buoyant density. Electron microscopy of virus agglutinated with Con A showed a zone of Con A-glycoprotein complexes averaging 12-15 nm in thickness.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-13764136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-13975866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-13986422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-14171467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-14284321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-16559945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-4099080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-4108573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-4108614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-4111052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-4115401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-4317349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-4319782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-4330466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-4332144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-4333567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-4334774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-4335524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-4344193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-4563598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-4873173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-4925356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-5110427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-5286356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-5489229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4514301-5938890
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1308-12
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:4514301-Agglutination,
pubmed-meshheading:4514301-Animals,
pubmed-meshheading:4514301-Cells, Cultured,
pubmed-meshheading:4514301-Centrifugation, Density Gradient,
pubmed-meshheading:4514301-Concanavalin A,
pubmed-meshheading:4514301-Culture Media,
pubmed-meshheading:4514301-Friend murine leukemia virus,
pubmed-meshheading:4514301-Glycoproteins,
pubmed-meshheading:4514301-Isotope Labeling,
pubmed-meshheading:4514301-Lectins,
pubmed-meshheading:4514301-Methods,
pubmed-meshheading:4514301-Mice,
pubmed-meshheading:4514301-Mice, Inbred Strains,
pubmed-meshheading:4514301-Microscopy, Electron,
pubmed-meshheading:4514301-Sucrose,
pubmed-meshheading:4514301-Tritium,
pubmed-meshheading:4514301-Uridine,
pubmed-meshheading:4514301-Viral Plaque Assay
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pubmed:year |
1973
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pubmed:articleTitle |
Purification of oncornaviruses by agglutination with concanacalin A (murine leukemia virus-phytohemagglutinin-friend virus).
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pubmed:publicationType |
Journal Article
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