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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1973-4-5
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pubmed:abstractText |
Molecular hybridization between [(3)H]-poly(U) and unlabeled RNA prepared from sea urchin eggs and embryos has been used to contrast the subcellular localization as well as the size distribution of adenylylated maternal RNA preexisting in the unfertilized egg with that adenylylated as a function of fertilization. Evidence reported establishes that such preadenylylated genetic messages are predominantly located in the ovum's subribosomal fraction and that fertilization elicits a rapid reallocation of these latent transcripts into the zygote's ribosomal fraction. Examination of the size distribution of the adenylylated RNA further demonstrates that the unfertilized egg contains a substantial population of RNA transcripts of exceptionally high molecular weight that are used as primers for the 2-fold net synthesis of poly(A) that follows fertilization. The poly(A)-rich tracts are shown to be covalently bonded to RNA. Assessment of the poly(A) content of nuclear and cytoplasmic fractions suggests that the function of poly(A) is not confined to the transport of genetic messages from the nucleus.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4510284-13767412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4510284-19210966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4510284-4110479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4510284-4193236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4510284-4316213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4510284-4337237,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/4510284-4340753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4510284-4501121,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/4510284-4504372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4510284-4505674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4510284-4536742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4510284-4561958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4510284-4570496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4510284-4662399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4510284-5011971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4510284-5017913,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/4510284-5767312
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
406-11
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:4510284-Adenine Nucleotides,
pubmed-meshheading:4510284-Animals,
pubmed-meshheading:4510284-Cell Fractionation,
pubmed-meshheading:4510284-Cell Nucleus,
pubmed-meshheading:4510284-Centrifugation, Density Gradient,
pubmed-meshheading:4510284-Cytoplasm,
pubmed-meshheading:4510284-Female,
pubmed-meshheading:4510284-Fertilization,
pubmed-meshheading:4510284-Nucleic Acid Denaturation,
pubmed-meshheading:4510284-Nucleic Acid Hybridization,
pubmed-meshheading:4510284-Ovum,
pubmed-meshheading:4510284-Poly U,
pubmed-meshheading:4510284-Polynucleotides,
pubmed-meshheading:4510284-RNA,
pubmed-meshheading:4510284-RNA, Ribosomal,
pubmed-meshheading:4510284-Ribosomes,
pubmed-meshheading:4510284-Sea Urchins,
pubmed-meshheading:4510284-Transcription, Genetic,
pubmed-meshheading:4510284-Tritium
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pubmed:year |
1973
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pubmed:articleTitle |
Cytoplasmic adenylylation and processing of maternal RNA.
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pubmed:publicationType |
Journal Article
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