Linked Life Data

4509656

Source:http://linkedlifedata.com/resource/pubmed/id/4509656

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1973-3-15
pubmed:abstractText
Pactamycin does not inhibit the overall initiation factor- and GTP-dependent binding of [(35)S]Met-tRNA(f) to rabbit reticulocyte ribosomes but does prevent the formation of Met-puromycin, provided that the antibiotic is present during the course of the binding reaction. These data indicate that pactamycin blocks the synthesis of a functional peptide-chain initiation complex. Sucrose density gradient centrifugation analysis of binding reactions shows that pactamycin causes the accumulation of an initiation complex on the smaller ribosomal subunit (smaller initiation complex), to which the 60S ribosomal subunit either cannot join or with which it forms a larger inactive 80S initiation complex that falls apart under the conditions used for isolation. The smaller initiation complex formed in the presence of pactamycin differs from the normal intermediate in peptide-chain initiation in being much more resistant to degradation by pancreatic RNase. In the presence of pactamycin, the inactive smaller complex can also form on mRNA to which an unaffected ribosomal couple is already attached, forming an oligoribosome lacking a larger ribosomal subunit or a "1.5 mer." These effects of pactamycin can be overcome to a considerable degree by elevation of the Mg(2+) concentration.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/4509656-4099511, http://linkedlifedata.com/resource/pubmed/commentcorrection/4509656-4330946, http://linkedlifedata.com/resource/pubmed/commentcorrection/4509656-4331648, http://linkedlifedata.com/resource/pubmed/commentcorrection/4509656-4501585, http://linkedlifedata.com/resource/pubmed/commentcorrection/4509656-4553693, http://linkedlifedata.com/resource/pubmed/commentcorrection/4509656-4909890, http://linkedlifedata.com/resource/pubmed/commentcorrection/4509656-4944310, http://linkedlifedata.com/resource/pubmed/commentcorrection/4509656-5015233, http://linkedlifedata.com/resource/pubmed/commentcorrection/4509656-5041910, http://linkedlifedata.com/resource/pubmed/commentcorrection/4509656-5114993, http://linkedlifedata.com/resource/pubmed/commentcorrection/4509656-5126935, http://linkedlifedata.com/resource/pubmed/commentcorrection/4509656-5288768, http://linkedlifedata.com/resource/pubmed/commentcorrection/4509656-5459119, http://linkedlifedata.com/resource/pubmed/commentcorrection/4509656-5960399
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
22-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1973
pubmed:articleTitle
Inhibition of reticulocyte peptide-chain initiation by pactamycin: accumulation of inactive ribosomal initiation complexes.
pubmed:publicationType
Journal Article