| pubmed-article:4462587 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:4462587 | lifeskim:mentions | umls-concept:C0006675 | lld:lifeskim |
| pubmed-article:4462587 | lifeskim:mentions | umls-concept:C0021852 | lld:lifeskim |
| pubmed-article:4462587 | lifeskim:mentions | umls-concept:C0000854 | lld:lifeskim |
| pubmed-article:4462587 | lifeskim:mentions | umls-concept:C0042866 | lld:lifeskim |
| pubmed-article:4462587 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
| pubmed-article:4462587 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
| pubmed-article:4462587 | lifeskim:mentions | umls-concept:C0205217 | lld:lifeskim |
| pubmed-article:4462587 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
| pubmed-article:4462587 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
| pubmed-article:4462587 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:4462587 | lifeskim:mentions | umls-concept:C1707520 | lld:lifeskim |
| pubmed-article:4462587 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:4462587 | pubmed:dateCreated | 1975-9-3 | lld:pubmed |
| pubmed-article:4462587 | pubmed:abstractText | 1. The synthesis of calcium-binding protein, a protein produced in the small intestine in response to vitamin D, was investigated with a view to determining whether calcium-binding-protein production could be correlated with the stimulation of calcium absorption by vitamin D. 2. A radioimmunological assay, which can quantitatively estimate calcium-binding-protein concentrations as low as 1mug/g wet wt., was used to detect the synthesis of soluble calcium-binding protein. 3. When used on intestinal supernatants from chicks dosed with vitamin D, calcium-binding protein was not detectable at 8h but was present after 12h at a concentration of 8.6mug/g wet wt.; in agreement with this an increase in calcium absorption due to vitamin D was detected at 12h but not at 8h. 4. The synthesis of calcium-binding protein was also monitored directly by making use of the ability of the iodinated antiserum to bind specifically to nascent calcium-binding protein chains on intestinal polyribosomes; in this way calcium-binding-protein synthesis could be detected 8h after dosage with vitamin D. Further, the binding reaction indicated a near linear increase in the calcium-binding-protein-synthesizing capacity over a 16h period. 5. From the amount of calcium-binding protein present 12 and 24h after vitamin D administration it is calculated that calcium-binding-protein mRNA is produced at approx. 1mol/min per intestinal cell. 6. It is concluded that the high correlation between the initiation of calcium-binding-protein synthesis and the stimulation of calcium absorption by vitamin D strengthens the proposal that calcium-binding protein plays an important role in calcium transport. | lld:pubmed |
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| pubmed-article:4462587 | pubmed:language | eng | lld:pubmed |
| pubmed-article:4462587 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:4462587 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:4462587 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:4462587 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:4462587 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:4462587 | pubmed:author | pubmed-author:EmtageJ SJS | lld:pubmed |
| pubmed-article:4462587 | pubmed:author | pubmed-author:KodicekEE | lld:pubmed |
| pubmed-article:4462587 | pubmed:author | pubmed-author:LawsonE MEM | lld:pubmed |
| pubmed-article:4462587 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:4462587 | pubmed:volume | 144 | lld:pubmed |
| pubmed-article:4462587 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:4462587 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:4462587 | pubmed:pagination | 339-46 | lld:pubmed |
| pubmed-article:4462587 | pubmed:dateRevised | 2010-9-10 | lld:pubmed |
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| pubmed-article:4462587 | pubmed:meshHeading | pubmed-meshheading:4462587-... | lld:pubmed |
| pubmed-article:4462587 | pubmed:year | 1974 | lld:pubmed |
| pubmed-article:4462587 | pubmed:articleTitle | The response of the small intestine to vitamin D. Correlation between calcium-binding-protein production and increased calcium absorption. | lld:pubmed |
| pubmed-article:4462587 | pubmed:publicationType | Journal Article | lld:pubmed |
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