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rdf:type |
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lifeskim:mentions |
umls-concept:C0000854,
umls-concept:C0006675,
umls-concept:C0021852,
umls-concept:C0033268,
umls-concept:C0042866,
umls-concept:C0205217,
umls-concept:C0871261,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C1707520,
umls-concept:C2911692
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pubmed:issue |
2
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pubmed:dateCreated |
1975-9-3
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pubmed:abstractText |
1. The synthesis of calcium-binding protein, a protein produced in the small intestine in response to vitamin D, was investigated with a view to determining whether calcium-binding-protein production could be correlated with the stimulation of calcium absorption by vitamin D. 2. A radioimmunological assay, which can quantitatively estimate calcium-binding-protein concentrations as low as 1mug/g wet wt., was used to detect the synthesis of soluble calcium-binding protein. 3. When used on intestinal supernatants from chicks dosed with vitamin D, calcium-binding protein was not detectable at 8h but was present after 12h at a concentration of 8.6mug/g wet wt.; in agreement with this an increase in calcium absorption due to vitamin D was detected at 12h but not at 8h. 4. The synthesis of calcium-binding protein was also monitored directly by making use of the ability of the iodinated antiserum to bind specifically to nascent calcium-binding protein chains on intestinal polyribosomes; in this way calcium-binding-protein synthesis could be detected 8h after dosage with vitamin D. Further, the binding reaction indicated a near linear increase in the calcium-binding-protein-synthesizing capacity over a 16h period. 5. From the amount of calcium-binding protein present 12 and 24h after vitamin D administration it is calculated that calcium-binding-protein mRNA is produced at approx. 1mol/min per intestinal cell. 6. It is concluded that the high correlation between the initiation of calcium-binding-protein synthesis and the stimulation of calcium absorption by vitamin D strengthens the proposal that calcium-binding protein plays an important role in calcium transport.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-14097352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-17797460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-4111967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-4112809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-4119369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-4267263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-4298516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-4298517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-4305086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-4309590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-4313404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-4321549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-4323313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-4324666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-4329876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-4455190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-4752955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-4901083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-5023786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4462587-5432063
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
144
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
339-46
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:4462587-Animals,
pubmed-meshheading:4462587-Biological Transport, Active,
pubmed-meshheading:4462587-Calcium,
pubmed-meshheading:4462587-Chemical Precipitation,
pubmed-meshheading:4462587-Chickens,
pubmed-meshheading:4462587-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:4462587-Immune Sera,
pubmed-meshheading:4462587-Intestinal Absorption,
pubmed-meshheading:4462587-Intestine, Small,
pubmed-meshheading:4462587-Iodine Radioisotopes,
pubmed-meshheading:4462587-Polyribosomes,
pubmed-meshheading:4462587-Protein Binding,
pubmed-meshheading:4462587-Protein Biosynthesis,
pubmed-meshheading:4462587-Radioimmunoassay,
pubmed-meshheading:4462587-Sodium Dodecyl Sulfate,
pubmed-meshheading:4462587-Time Factors,
pubmed-meshheading:4462587-Vitamin D
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pubmed:year |
1974
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pubmed:articleTitle |
The response of the small intestine to vitamin D. Correlation between calcium-binding-protein production and increased calcium absorption.
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pubmed:publicationType |
Journal Article
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