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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1975-6-26
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pubmed:abstractText |
1. The mechanism of depolarization of squid axon membranes caused by grayanotoxin I has been studied by means of internal perfusion and voltage clamp techniques.2. The depolarization induced by either internal or external application of grayanotoxin I was reversed by decreasing the external sodium concentration from 449 to 1 mm.3. No depolarization was observed when both external and internal media were devoid of sodium ions, indicating that the depolarization by grayanotoxin I in normal media is due to a specific increase in resting sodium permeability.4. The resting sodium permeability as measured by voltage clamp was increased to 1.31 x 10(-6) cm/sec by internal application of 1 x 10(-5)m grayanotoxin I, an increase by a factor of about 90.5. The apparent dissociation constant of internally applied grayanotoxin I in increasing the resting sodium permeability was estimated to be 4.12 x 10(-5)m, and the toxin interacts with the membrane receptor on a one-to-one stoichiometric basis.6. Tetrodotoxin antagonized the action of grayanotoxin I in increasing the resting sodium permeability in a non-competitive manner.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-13824559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-13969165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-14232131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-14328451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-14946713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-18128147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-19873371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-4341317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-4401111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-4646588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-4686003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-4686004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-4688172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-4688173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-4903416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-5038273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-5312067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-5440891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-5520505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-5564762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-5575342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-5704715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-5812424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-5814950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-5940896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-6032201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-6033593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-6059668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455829-6070696
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
242
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
471-87
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:4455829-Animals,
pubmed-meshheading:4455829-Antitoxins,
pubmed-meshheading:4455829-Cell Membrane,
pubmed-meshheading:4455829-Cell Membrane Permeability,
pubmed-meshheading:4455829-Culture Media,
pubmed-meshheading:4455829-Decapodiformes,
pubmed-meshheading:4455829-Dose-Response Relationship, Drug,
pubmed-meshheading:4455829-Electric Conductivity,
pubmed-meshheading:4455829-Membrane Potentials,
pubmed-meshheading:4455829-Neuromuscular Depolarizing Agents,
pubmed-meshheading:4455829-Neurons,
pubmed-meshheading:4455829-Plant Extracts,
pubmed-meshheading:4455829-Plants,
pubmed-meshheading:4455829-Sodium,
pubmed-meshheading:4455829-Tetrodotoxin,
pubmed-meshheading:4455829-Toxins, Biological
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pubmed:year |
1974
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pubmed:articleTitle |
Mechanism of nerve membrane depolarization caused by grayanotoxin I.
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pubmed:publicationType |
Journal Article,
In Vitro
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