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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1975-5-29
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pubmed:abstractText |
The synthesis of peptidoglycan by cell-free membrane and membrane+wall preparations from an autolysin-deficient, beta-lactamase-negative mutant of Bacillus licheniformis N.C.T.C. 6346 was studied. The membrane preparation synthesized un-cross-linked polymer, the formation of which was not inhibited by beta-lactam antibiotics. Release of d-alanine by the action of d-alanine carboxypeptidase was inhibited variably according to the antibiotic. This inhibition was reversed by neutral hydroxylamine but not by the action of beta-lactamases or by washing. Bacitracin inhibited peptidoglycan synthesis, but not the d-alanine carboxypeptidase. Examination of peptidoglycan synthesized in the presence of excess of bacitracin showed that synthesis was not restricted to the addition of one disaccharide-pentapeptide unit at each synthetic site, an average of 2-3 disaccharide-pentapeptide units being added. Peptidoglycan synthesis was three- to four-fold more sensitive to vancomycin than was the release of d-alanine by the action of the carboxypeptidase. Incorporation of newly synthesized peptidoglycan into pre-existing cell wall was studied in membrane+wall preparations. This incorporation was catalysed by a benzylpenicillin- and cephaloridine-sensitive transpeptidase. The concentrations of these antibiotics giving 50% inhibition of incorporation were almost identical with those required to inhibit growth of the bacillus. Inhibition of the transpeptidase was reversed by treatment with beta-lactamase or by washing.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-13271455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-13789369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-13835293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-14324547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-14416360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-16591529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-4198851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-4248525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-4255188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-4255189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-4259358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-4262449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-4262450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-4321754,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-4343965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-4568761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-4570613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-4621974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-4627447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-4627581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-4722034,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-5231417,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/4455202-6027794
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
141
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
227-41
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:4455202-Alanine,
pubmed-meshheading:4455202-Anti-Bacterial Agents,
pubmed-meshheading:4455202-Bacillus,
pubmed-meshheading:4455202-Bacitracin,
pubmed-meshheading:4455202-Carboxypeptidases,
pubmed-meshheading:4455202-Cell Membrane,
pubmed-meshheading:4455202-Cell Wall,
pubmed-meshheading:4455202-Cephalosporins,
pubmed-meshheading:4455202-Chromatography, Paper,
pubmed-meshheading:4455202-Electrophoresis, Paper,
pubmed-meshheading:4455202-Hydroxylamines,
pubmed-meshheading:4455202-Mutation,
pubmed-meshheading:4455202-Penicillins,
pubmed-meshheading:4455202-Peptidoglycan,
pubmed-meshheading:4455202-Temperature,
pubmed-meshheading:4455202-Vancomycin
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pubmed:year |
1974
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pubmed:articleTitle |
The synthesis of peptidoglycan in an autolysin-deficient mutant of Bacillus licheniformis N.C.T.C. 6346 and the effect of beta-lactam antibiotics, bacitracin and vancomycin.
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pubmed:publicationType |
Journal Article
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