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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1974-12-10
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pubmed:abstractText |
Glucosylsphingosine has been isolated for the first time as a natural constituent from Gaucher's spleen. On thin-layer chromatography, it migrates with authentic glucosylsphingosine, yielding a positive color reaction with ninhydrin for the amino group and with alpha-naphthol-sulfuric acid for the carbohydrate residue. N-Acylation with palmitic acid gave rise to glucosylceramide, which was cleaved by purified glucosylceramide: beta-glucosidase to ceramide. Gas-liquid chromatography of the trimethylsilyl derivative showed a retention time similar to authentic glucosylsphingosine. Gas-liquid chromatographic analysis of the trimethylsilyl derivatives after methanolysis revealed the presence of only glucose and C(18)-sphingosine. Mass spectral data further supported the structural identity with glucosylsphingosine.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-2275
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
484-90
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:4414001-Acylation,
pubmed-meshheading:4414001-Adult,
pubmed-meshheading:4414001-Autopsy,
pubmed-meshheading:4414001-Ceramides,
pubmed-meshheading:4414001-Cerebrosides,
pubmed-meshheading:4414001-Chemical Phenomena,
pubmed-meshheading:4414001-Chemistry,
pubmed-meshheading:4414001-Chromatography, Gas,
pubmed-meshheading:4414001-Chromatography, Ion Exchange,
pubmed-meshheading:4414001-Chromatography, Thin Layer,
pubmed-meshheading:4414001-Gaucher Disease,
pubmed-meshheading:4414001-Glucose,
pubmed-meshheading:4414001-Glycoside Hydrolases,
pubmed-meshheading:4414001-Glycosides,
pubmed-meshheading:4414001-Humans,
pubmed-meshheading:4414001-Hydrolysis,
pubmed-meshheading:4414001-Mass Spectrometry,
pubmed-meshheading:4414001-Palmitic Acids,
pubmed-meshheading:4414001-Sphingosine,
pubmed-meshheading:4414001-Spleen
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pubmed:year |
1974
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pubmed:articleTitle |
Isolation and characterization of glucosylsphingosine from Gaucher's spleen.
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pubmed:publicationType |
Journal Article,
Comparative Study
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