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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1979-7-25
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pubmed:abstractText |
Protamine-125I-insulin with low specific radioactivity was prepared using 125, 127I-insulin, 0.2 I/mole. The preparations were characterized by disc electrophoresis, isoelectric focusing and analytical gel chromatography in order to evaluate the suitability of 125I-insulin as marker for insulin in protamine-insulin. The stability of the preparations was followed up to 90 days at 4 degrees C. The biological and immunological activity was determined in mice, isolated rat fat cells and by radioimmunoassay. It was concluded that both from a chemical and a biological point of view, the protamine-125I-insulin is a satisfactory preparation that might be used in absorption studies.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0018-5043
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
85-90
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:437686-Absorption,
pubmed-meshheading:437686-Chromatography, Gel,
pubmed-meshheading:437686-Diabetes Mellitus,
pubmed-meshheading:437686-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:437686-Humans,
pubmed-meshheading:437686-Insulin,
pubmed-meshheading:437686-Iodine Radioisotopes,
pubmed-meshheading:437686-Isoelectric Point,
pubmed-meshheading:437686-Protamines,
pubmed-meshheading:437686-Radioactive Tracers
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pubmed:year |
1979
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pubmed:articleTitle |
Absorption of protamine-insulin in diabetic patients. I. Preparation and characterization of protamine-125I-insulin.
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pubmed:publicationType |
Journal Article
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