|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1974-10-31
|
|
pubmed:abstractText |
Functional and morphologic effects of cytochalasin B on the cultivated macrophage were examined to determine the basis for plasma membrane movements of the type required for endocytosis and/or spreading on a substratum. Inhibition of phagocytosis and changes in cell shape by cytochalasin B exhibited nearly identical dose-response curves requiring 2-5 x 10(-6) M and 1-2 x 10(-5) M cytochalasin B to inhibit these functions by 50% and 100%, respectively. In contrast, hexose transport was ten times more sensitive to the drug requiring 2-3 x 10(-7) M cytochalasin B to achieve 50% inhibition of 2-deoxyglucose uptake. Inhibition of phagocytosis and changes in cell shape could not be explained solely by drug effects on hexose transport. Analysis of serial thin sections showed that cytochalasin B doses inhibitory for hexose transport had no effect on distribution or organization of either of the two subplasmalemmal microfilament types. However, cytochalasin B concentrations (2.0 x 10(-5) M) that inhibited phagocytosis and altered cell shape disorganized and/or disrupted oriented bundles of 40-50-A subplasmalemmal microfilaments, but had no effect on the microfilamentous network. Comparative dose-response studies showing positive correlations among cytochalasin B effects on phagocytosis, changes in cell shape, and alterations in oriented subplasmalemmal microfilament bundles provide additional support for the hypothesis that microfilamentous structures play a role in translocation of plasma membrane required for endocytosis and cell motility.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4368822-13634058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4368822-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4368822-4257818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4368822-4289715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4368822-4329157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4368822-4356569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4368822-4881437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4368822-4932046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4368822-4933470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4368822-4937093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4368822-5054338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4368822-5132788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4368822-5538822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4368822-6067685
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0021-9525
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
62
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
647-59
|
|
pubmed:dateRevised |
2010-6-22
|
|
pubmed:meshHeading |
pubmed-meshheading:4368822-Animals,
pubmed-meshheading:4368822-Cell Membrane,
pubmed-meshheading:4368822-Cell Movement,
pubmed-meshheading:4368822-Cytochalasin B,
pubmed-meshheading:4368822-Dose-Response Relationship, Drug,
pubmed-meshheading:4368822-Glucose,
pubmed-meshheading:4368822-Hexoses,
pubmed-meshheading:4368822-Inclusion Bodies,
pubmed-meshheading:4368822-Macrophages,
pubmed-meshheading:4368822-Male,
pubmed-meshheading:4368822-Mice,
pubmed-meshheading:4368822-Microscopy, Electron, Scanning,
pubmed-meshheading:4368822-Microscopy, Phase-Contrast,
pubmed-meshheading:4368822-Phagocytosis
|
|
pubmed:year |
1974
|
|
pubmed:articleTitle |
Inhibition of phagocytosis and plasma membrane mobility of the cultivated macrophage by cytochalasin B. Role of subplasmalemmal microfilaments.
|
|
pubmed:publicationType |
Journal Article
|
