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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1974-6-4
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pubmed:abstractText |
1. cis-Benzene glycol dehydrogenase was purified to a homogeneous state from a species of Pseudomonas grown with benzene as the major carbon source. 2. The enzyme was specific for the cis-isomer of its substrate and required NAD(+) as hydrogen acceptor. 3. Partial inactivation of the enzyme, which was observed during purification, could be reversed by the addition of Fe(2+) and GSH. 4. A molecular weight of 440000 was calculated from data obtained by sedimentation-velocity and diffusion analysis in the ultracentrifuge. Sodium dodecyl sulphate polyacrylamide-gel electrophoresis indicated a subunit of molecular weight 110000. 5. p-Chloromercuribenzoic acid and 1,10-phenanthroline were shown to inhibit the enzyme.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
136
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
927-34
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:4362337-Alcohol Oxidoreductases,
pubmed-meshheading:4362337-Benzene,
pubmed-meshheading:4362337-Cell-Free System,
pubmed-meshheading:4362337-Chloromercuribenzoates,
pubmed-meshheading:4362337-Chromatography, DEAE-Cellulose,
pubmed-meshheading:4362337-Cyclohexanols,
pubmed-meshheading:4362337-Diffusion,
pubmed-meshheading:4362337-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:4362337-Enzyme Activation,
pubmed-meshheading:4362337-Glutathione,
pubmed-meshheading:4362337-Hydrogen-Ion Concentration,
pubmed-meshheading:4362337-Iron,
pubmed-meshheading:4362337-Isomerism,
pubmed-meshheading:4362337-Molecular Weight,
pubmed-meshheading:4362337-NAD,
pubmed-meshheading:4362337-Oxidoreductases,
pubmed-meshheading:4362337-Phenanthrolines,
pubmed-meshheading:4362337-Pseudomonas,
pubmed-meshheading:4362337-Sodium Dodecyl Sulfate,
pubmed-meshheading:4362337-Ultracentrifugation
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pubmed:year |
1973
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pubmed:articleTitle |
The metabolism of benzene by bacteria. Purification and some properties of the enzyme cis-1,2-dihydroxycyclohexa-3,5-diene (nicotinamide adenine dinucleotide) oxidoreductase (cis-benzene glycol dehydrogenase).
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pubmed:publicationType |
Journal Article
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