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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4-5
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pubmed:dateCreated |
1979-7-28
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|
pubmed:abstractText |
Unscheduled DNA synthesis was induced by 3,3'4,4'-tetrachloroazobenzene (TCAB)) in freshly isolated suspensions of rat hepatocytes. A dose-dependent response was demonstrated. Hepatocellular DNA was obtained after the chloroform-isoamyl alchohol-phenol extraction of the isolated nuclei. The induction of unscheduled DNA synthesis was measured by the incorporation of [3H]-thymidine in the presence of hydroxyurea as determined by the scintillation counting assay. DNA repair data obtained in this study on benzo[a]pyrene and methyl methanesulfonate are comparable to a previous report using primary cultures of hepatocytes and cesium chloride gradients. Hence, the present method offers promise as a rapid and sensitive screen for chemical carcinogens.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0304-3835
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
207-12
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:436117-Animals,
pubmed-meshheading:436117-Azo Compounds,
pubmed-meshheading:436117-Benzopyrenes,
pubmed-meshheading:436117-Carcinogens,
pubmed-meshheading:436117-Chlorobenzenes,
pubmed-meshheading:436117-DNA,
pubmed-meshheading:436117-DNA Repair,
pubmed-meshheading:436117-Drug Evaluation, Preclinical,
pubmed-meshheading:436117-Liver,
pubmed-meshheading:436117-Male,
pubmed-meshheading:436117-Methyl Methanesulfonate,
pubmed-meshheading:436117-Rats
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pubmed:year |
1979
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pubmed:articleTitle |
Induction of unscheduled DNA synthesis in suspensions of rat hepatocytes by an environmental toxicant, 3,3'4,4'-tetrachloroazobenzene.
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
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