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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1973-10-9
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pubmed:abstractText |
We present evidence that virus-specific RNA is present in polyribosomes of transformed cells replicating the murine sarcoma-leukemia virus complex and that it serves as messenger RNA for the synthesis of viral-coded proteins. Both virus-specific RNA (detected by hybridization with the [(3)H]DNA product of the viral RNA-directed DNA polymerase) and nascent viral polypeptides (measured by precipitation with antiserum to purified virus) were found in membrane-bound and free polyribosomes. Membrane-bound polyribosomes contained a higher content of both virus-specific RNA and nascent viral polypeptides. From 60 to 70% of viral RNA sequences were released from polyribosomes with EDTA, consistent with a function as messenger RNA. Maximum amounts of both virus-specific RNA and nascent viral polypeptides were found in the polyribosome region sedimenting at about 350 S.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-14446510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-4194867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-4292778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-4332006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-4335063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-4337163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-4338254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-4338637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-4342052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-4507519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-5000315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-5023666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-5166922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-5220869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-5263771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-5266177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-5277091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-5280402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-5284428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-5484455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-6034985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4352969-6052649
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2064-8
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:4352969-Animals,
pubmed-meshheading:4352969-Cell Fractionation,
pubmed-meshheading:4352969-Cell Line,
pubmed-meshheading:4352969-Cell Transformation, Neoplastic,
pubmed-meshheading:4352969-DNA, Viral,
pubmed-meshheading:4352969-Gammaretrovirus,
pubmed-meshheading:4352969-Immunoassay,
pubmed-meshheading:4352969-Mice,
pubmed-meshheading:4352969-Nucleic Acid Hybridization,
pubmed-meshheading:4352969-Peptides,
pubmed-meshheading:4352969-Polyribosomes,
pubmed-meshheading:4352969-Precipitin Tests,
pubmed-meshheading:4352969-RNA, Messenger,
pubmed-meshheading:4352969-RNA, Viral,
pubmed-meshheading:4352969-Tritium,
pubmed-meshheading:4352969-Viral Proteins,
pubmed-meshheading:4352969-Virus Replication
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pubmed:year |
1973
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pubmed:articleTitle |
Virus-specific messenger RNA and nascent polypeptides in polyribosomes of cells replicating murine sarcoma-leukemia viruses.
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pubmed:publicationType |
Journal Article
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