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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1973-8-6
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pubmed:abstractText |
Elucidation of the functions of the eosinophil might be accomplished by analysis of the granule constituents. We have purified eosinophils (93% or greater) from the peritoneal cavity of the guinea pig and have investigated a variety of methods to disrupt cells and liberate intact granules. Lysis in 0.34 M sucrose gave the best yield of granules and these had the characteristic morphology of eosinophil granules when examined by electron microscopy. Granules were solubilized by a variety of treatments and the solutions analyzed by polyacrylamide electrophoresis at pH 3 in 6 M urea. Comparison of the electrophoretic patterns of solubilized eosinophil and neutrophil granules revealed a difference: a major portion (53+/-3%; x +/-1 SE) of the protein from the eosinophil granule migrated as a single component. This major band protein has a molecular weight between 6,000 and 12,000 daltons and a pI of 10 or greater. Analysis of eosinophil granule constituents on Sephadex G-50 revealed two main peaks; peak 1 possessed peroxidase activity and peak 2 contained the major band protein. These studies indicate that eosinophil granules contain a cationic protein of low molecular weight which lacks peroxidase activity and which accounts for greater than 50% of granule protein.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4350835-13068370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4350835-13315511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4350835-13694490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4350835-13762726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4350835-14074391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4350835-14247717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4350835-14491328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4350835-14944305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4350835-4175322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4350835-4181945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4350835-4326772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4350835-4623114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4350835-4871738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4350835-4979520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4350835-5778650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4350835-5806584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4350835-5926298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4350835-5934974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4350835-5962951
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
137
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1459-71
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pubmed:dateRevised |
2010-6-22
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pubmed:meshHeading |
pubmed-meshheading:4350835-Animals,
pubmed-meshheading:4350835-Biological Assay,
pubmed-meshheading:4350835-Blood Proteins,
pubmed-meshheading:4350835-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:4350835-Eosinophils,
pubmed-meshheading:4350835-Freeze Drying,
pubmed-meshheading:4350835-Guinea Pigs,
pubmed-meshheading:4350835-Inclusion Bodies,
pubmed-meshheading:4350835-Microscopy, Electron,
pubmed-meshheading:4350835-Peroxidases
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pubmed:year |
1973
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pubmed:articleTitle |
Identification of a major basic protein in guinea pig eosinophil granules.
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pubmed:publicationType |
Journal Article
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