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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1973-6-19
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pubmed:abstractText |
A combination of differential centrifugation and carrier-free continuous electrophoresis is introduced as a new method for the isolation of animal cell organelles. Various buffers were systematically checked in order to find the system which preserves the organelles and gives as well a good separation in the free-flow electrophoresis apparatus. Triethanolamine-acetate buffer (10 mM), pH 7.4 was used. The isolated lysosomes were pure according to marker enzymes and electron micrographs. A heterogeneity of the lysosomes in electrophoretic mobility was demonstrated with respect to the marker enzymes arylsulfatase and beta-glucuronidase. The lysosomes with higher mobility showed a maximum enrichment of 240-fold with respect to arylsulfatase. The lysosomes with lower electrophoretic mobility showed a 65-fold enrichment with respect to beta-glucuronidase. The ratio of beta-glucuronidase to arylsulfatase varied from 2:1 to 1:2 in lysosomes of different mobility. The yield amounted to approximately 1 mg of lysosomal protein per gram of liver protein. 5-8 mg of lysosomes can be obtained in one experiment. The electrophoretic separation proves to be an effective tool in obtaining pure and well preserved lysosomes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-13249955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-13797961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-13797962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-13961760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-14159305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-14186728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-14203144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-14207278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-14304877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-14317282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-14324936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-14832284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-14938361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-16749142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-4291912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-4298925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-4318844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-4378797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-4379260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-5638866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-5677004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-5867784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-6050757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-6066294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349132-6075421
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acid Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/Buffers,
http://linkedlifedata.com/resource/pubmed/chemical/Catalase,
http://linkedlifedata.com/resource/pubmed/chemical/Electron Transport Complex IV,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose-6-Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/Glucuronidase,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/Monoamine Oxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfatases,
http://linkedlifedata.com/resource/pubmed/chemical/Tissue Extracts
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
46
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
576-91
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pubmed:dateRevised |
2010-6-22
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pubmed:meshHeading |
pubmed-meshheading:4349132-Acid Phosphatase,
pubmed-meshheading:4349132-Buffers,
pubmed-meshheading:4349132-Catalase,
pubmed-meshheading:4349132-Cell Fractionation,
pubmed-meshheading:4349132-Centrifugation,
pubmed-meshheading:4349132-Centrifugation, Density Gradient,
pubmed-meshheading:4349132-Cytoplasmic Granules,
pubmed-meshheading:4349132-Electron Transport Complex IV,
pubmed-meshheading:4349132-Electrophoresis,
pubmed-meshheading:4349132-Glucose-6-Phosphatase,
pubmed-meshheading:4349132-Glucuronidase,
pubmed-meshheading:4349132-Histocytochemistry,
pubmed-meshheading:4349132-Hydrogen-Ion Concentration,
pubmed-meshheading:4349132-Hydrolases,
pubmed-meshheading:4349132-Liver,
pubmed-meshheading:4349132-Lysosomes,
pubmed-meshheading:4349132-Microscopy, Electron,
pubmed-meshheading:4349132-Microscopy, Phase-Contrast,
pubmed-meshheading:4349132-Microsomes,
pubmed-meshheading:4349132-Mitochondria,
pubmed-meshheading:4349132-Monoamine Oxidase,
pubmed-meshheading:4349132-Proteins,
pubmed-meshheading:4349132-Sulfatases,
pubmed-meshheading:4349132-Tissue Extracts
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pubmed:year |
1970
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pubmed:articleTitle |
A new method for the preparation of rat liver lysosomes. Separation of cell organelles of rat liver by carrier-free continuous electrophoresis.
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pubmed:publicationType |
Journal Article
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