Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1972-4-6
pubmed:abstractText
A method for culturing non- or slowly growing, differentiated fetal rat liver cells is described. It involves the use of collagenase as a digesting agent and of a selective medium deficient in arginine which suppresses the growth of nonparenchymal liver cells. Evidence is presented that surviving cells (a) retain liver-specific urea cycle functions measured by their capacity to transform ornithine into arginine, (b) synthesize DNA in glucose-deficient medium, and (c) synthesize and secrete albumin. This primary cell culture responds to partially hepatectomized rat serum and may be an appropriate assay system for the study of mechanisms which regulate liver regeneration.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
559-68
pubmed:dateRevised
2010-6-22
pubmed:meshHeading
pubmed-meshheading:4333210-Animals, pubmed-meshheading:4333210-Arginine, pubmed-meshheading:4333210-Blood, pubmed-meshheading:4333210-Cell Differentiation, pubmed-meshheading:4333210-Cells, Cultured, pubmed-meshheading:4333210-Complement Fixation Tests, pubmed-meshheading:4333210-Culture Media, pubmed-meshheading:4333210-DNA, pubmed-meshheading:4333210-Female, pubmed-meshheading:4333210-Fetus, pubmed-meshheading:4333210-Fibroblasts, pubmed-meshheading:4333210-Hepatectomy, pubmed-meshheading:4333210-Liver, pubmed-meshheading:4333210-Liver Regeneration, pubmed-meshheading:4333210-Methods, pubmed-meshheading:4333210-Microbial Collagenase, pubmed-meshheading:4333210-Microscopy, Phase-Contrast, pubmed-meshheading:4333210-Ornithine, pubmed-meshheading:4333210-Pregnancy, pubmed-meshheading:4333210-Rats, pubmed-meshheading:4333210-Serum Albumin, pubmed-meshheading:4333210-Simian virus 40, pubmed-meshheading:4333210-Thymidine, pubmed-meshheading:4333210-Tritium
pubmed:year
1972
pubmed:articleTitle
Studies on primary cultures of differentiated fetal liver cells.
pubmed:publicationType
Journal Article, In Vitro