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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1972-3-8
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pubmed:abstractText |
A method was developed for freeze-dry stabilization of poliovirus type 3. An ultrafiltration procedure was used to remove salts from infected tissue culture fluid, and the virus was freeze-dried after suspension in an alkaline organic buffer. This method was further tested with other picornaviruses including poliovirus types 1 and 2, coxsackieviruses A9, A20, B2, and B5, echovirus 11, and the encephalomyocarditis virus. Freeze-dried preparations of the poliovirus could be shipped to distant laboratories at ambient temperature with excellent retention of infectivity. Data are presented showing effects of freeze-drying as well as results of exposure to temperatures to 37 C.
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pubmed:commentsCorrections | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
|
pubmed:issn |
0003-6919
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
850-3
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:4332042-Buffers,
pubmed-meshheading:4332042-Culture Media,
pubmed-meshheading:4332042-Culture Techniques,
pubmed-meshheading:4332042-Encephalomyocarditis virus,
pubmed-meshheading:4332042-Enterovirus,
pubmed-meshheading:4332042-Filtration,
pubmed-meshheading:4332042-Freeze Drying,
pubmed-meshheading:4332042-Methods,
pubmed-meshheading:4332042-Tromethamine,
pubmed-meshheading:4332042-Virus Cultivation
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pubmed:year |
1971
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pubmed:articleTitle |
Preservation of enteroviruses by freeze-drying.
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pubmed:publicationType |
Journal Article
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