Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1971-3-12
pubmed:abstractText
1) Both the growth rate and the maximum population density of several normal, virus-transformed, and cancer cells were markedly pH-dependent; the optimum varied from pH 6.9 to 7.8. At the optimum pH, some diploid human cells attained population densities comparable to those of cancer or virus-transformed cells. Contact inhibition of growth is facilitated by repeated fluctuations of pH in nonphysiological ranges, and may not be an intrinsic and necessary attribute of diploid cells in culture. 2) At pH 8.3, at which there was little or no cellular multiplication, the protein content per cell increased 2- to 5-fold over a period of 10-16 days, and was slowly reversed to normal concentrations on restoration of pH to the optimal range. 3) Uridine uptake by contact-inhibited human cell cultures was stimulated by refeeding with salt solution, and to the same extent as by complete (serum-supplemented) growth medium; that immediate increase did not involve the reinitiation of cellular growth and multiplication. Contact inhibition was, however, reversed in 2-4 days by an appropriate increase in the serum concentration of the medium.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/4322262-13297784, http://linkedlifedata.com/resource/pubmed/commentcorrection/4322262-13675766, http://linkedlifedata.com/resource/pubmed/commentcorrection/4322262-13985244, http://linkedlifedata.com/resource/pubmed/commentcorrection/4322262-14294068, http://linkedlifedata.com/resource/pubmed/commentcorrection/4322262-14315085, http://linkedlifedata.com/resource/pubmed/commentcorrection/4322262-14392239, http://linkedlifedata.com/resource/pubmed/commentcorrection/4322262-4313331, http://linkedlifedata.com/resource/pubmed/commentcorrection/4322262-4317881, http://linkedlifedata.com/resource/pubmed/commentcorrection/4322262-4894298, http://linkedlifedata.com/resource/pubmed/commentcorrection/4322262-5241531, http://linkedlifedata.com/resource/pubmed/commentcorrection/4322262-5248816, http://linkedlifedata.com/resource/pubmed/commentcorrection/4322262-5257005, http://linkedlifedata.com/resource/pubmed/commentcorrection/4322262-5419270, http://linkedlifedata.com/resource/pubmed/commentcorrection/4322262-5884360, http://linkedlifedata.com/resource/pubmed/commentcorrection/4322262-5884626, http://linkedlifedata.com/resource/pubmed/commentcorrection/4322262-6029791
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
229-33
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:4322262-Adenoviridae, pubmed-meshheading:4322262-Animals, pubmed-meshheading:4322262-Bicarbonates, pubmed-meshheading:4322262-Blood, pubmed-meshheading:4322262-Cell Line, pubmed-meshheading:4322262-Cell Transformation, Neoplastic, pubmed-meshheading:4322262-Contact Inhibition, pubmed-meshheading:4322262-Culture Media, pubmed-meshheading:4322262-Culture Techniques, pubmed-meshheading:4322262-Cytopathogenic Effect, Viral, pubmed-meshheading:4322262-Diploidy, pubmed-meshheading:4322262-Fibroblasts, pubmed-meshheading:4322262-Haplorhini, pubmed-meshheading:4322262-HeLa Cells, pubmed-meshheading:4322262-Humans, pubmed-meshheading:4322262-Hydrogen-Ion Concentration, pubmed-meshheading:4322262-Kidney, pubmed-meshheading:4322262-Lens, Crystalline, pubmed-meshheading:4322262-Lung, pubmed-meshheading:4322262-Neoplasm Proteins, pubmed-meshheading:4322262-Simian virus 40, pubmed-meshheading:4322262-Skin, pubmed-meshheading:4322262-Uridine
pubmed:year
1971
pubmed:articleTitle
pH as a determinant of cellular growth and contact inhibition.
pubmed:publicationType
Journal Article, In Vitro