| pubmed-article:4318949 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:4318949 | lifeskim:mentions | umls-concept:C0999948 | lld:lifeskim |
| pubmed-article:4318949 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:4318949 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:4318949 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
| pubmed-article:4318949 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:4318949 | pubmed:dateCreated | 1970-11-6 | lld:pubmed |
| pubmed-article:4318949 | pubmed:abstractText | NaClO(4) was employed in a technique for the rapid extraction of reovirus ribonucleic acid (RNA). The extracted RNA, which was purified in a Cs(2)SO(4) equilibrium density gradient, had a buoyant density of 1.61 g/cm(3) and a sedimentation coefficient of 15S in a 7 to 20% sucrose gradient. It was 90% resistant to ribonuclease in a solution of high ionic strength (0.1 m NaCl). The sensitivity of reovirus RNA to ribonuclease increased with decreasing ionic strength. The thermal denaturation transition of the RNA began at 78 C and was complete at 85 C. The T(m) of the transition was 81 C in 0.01 m tris(hydroxymethyl)aminomethane buffer (pH 7.2) containing 0.001 m ethylenediaminetetraacetate. Thermal denaturation of reovirus RNA resulted in the formation of three ribonuclease-sensitive fractions. Denaturation at 25 C in the presence of dimethyl sulfoxide resulted in the formation of two ribonuclease-sensitive fractions. | lld:pubmed |
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| pubmed-article:4318949 | pubmed:language | eng | lld:pubmed |
| pubmed-article:4318949 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:4318949 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:4318949 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:4318949 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:4318949 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:4318949 | pubmed:author | pubmed-author:FranklinR MRM | lld:pubmed |
| pubmed-article:4318949 | pubmed:author | pubmed-author:IglewskiW JWJ | lld:pubmed |
| pubmed-article:4318949 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:4318949 | pubmed:volume | 1 | lld:pubmed |
| pubmed-article:4318949 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:4318949 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:4318949 | pubmed:pagination | 302-7 | lld:pubmed |
| pubmed-article:4318949 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:4318949 | pubmed:meshHeading | pubmed-meshheading:4318949-... | lld:pubmed |
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| pubmed-article:4318949 | pubmed:meshHeading | pubmed-meshheading:4318949-... | lld:pubmed |
| pubmed-article:4318949 | pubmed:meshHeading | pubmed-meshheading:4318949-... | lld:pubmed |
| pubmed-article:4318949 | pubmed:meshHeading | pubmed-meshheading:4318949-... | lld:pubmed |
| pubmed-article:4318949 | pubmed:meshHeading | pubmed-meshheading:4318949-... | lld:pubmed |
| pubmed-article:4318949 | pubmed:meshHeading | pubmed-meshheading:4318949-... | lld:pubmed |
| pubmed-article:4318949 | pubmed:meshHeading | pubmed-meshheading:4318949-... | lld:pubmed |
| pubmed-article:4318949 | pubmed:year | 1967 | lld:pubmed |
| pubmed-article:4318949 | pubmed:articleTitle | Purification and properties of reovirus ribonucleic acid. | lld:pubmed |
| pubmed-article:4318949 | pubmed:publicationType | Journal Article | lld:pubmed |
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