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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1970-11-6
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pubmed:abstractText |
NaClO(4) was employed in a technique for the rapid extraction of reovirus ribonucleic acid (RNA). The extracted RNA, which was purified in a Cs(2)SO(4) equilibrium density gradient, had a buoyant density of 1.61 g/cm(3) and a sedimentation coefficient of 15S in a 7 to 20% sucrose gradient. It was 90% resistant to ribonuclease in a solution of high ionic strength (0.1 m NaCl). The sensitivity of reovirus RNA to ribonuclease increased with decreasing ionic strength. The thermal denaturation transition of the RNA began at 78 C and was complete at 85 C. The T(m) of the transition was 81 C in 0.01 m tris(hydroxymethyl)aminomethane buffer (pH 7.2) containing 0.001 m ethylenediaminetetraacetate. Thermal denaturation of reovirus RNA resulted in the formation of three ribonuclease-sensitive fractions. Denaturation at 25 C in the presence of dimethyl sulfoxide resulted in the formation of two ribonuclease-sensitive fractions.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4318949-14016998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4318949-14243517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4318949-14244714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4318949-16591092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4318949-4158705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4318949-4160301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4318949-4289760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4318949-5217464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4318949-5623959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4318949-5865514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4318949-5938891
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
302-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1967
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pubmed:articleTitle |
Purification and properties of reovirus ribonucleic acid.
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pubmed:publicationType |
Journal Article
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