Linked Life Data

4270436

Source:http://linkedlifedata.com/resource/pubmed/id/4270436

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1973-12-16
pubmed:abstractText
The effects of a neonatal calf diarrhea virus on cell cultures were investigated. Bovine embryonic kidney cell cultures were the most satisfactory for production of virus. Cytoplasmic changes detected after inoculation with a high multiplicity of virus were: 1) cytoplasmic vacuoles; 2) some eosinophilic cytoplasmic inclusions; and 3) some degeneration of cells and detachment from the monolayer. Cultures stained with fluorescein-labeled antibody showed cytoplasmic fluorescence as early as four hr after infection with the maximum fluorescence at five days. No cross reactions were observed between the neonatal calf diarrhea virus and reovirus type 1 or type 3 by the fluorescent antibody technique. Plaques were small and were not produced consistently. The optimal adsorption time was one to two hr. The maximum titer was reached at 18 hr, with the cell-associated titer remaining higher than the cell-free titer until that time. An interferon was produced by cultures infected with either ultraviolet-inactivated or untreated virus.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0008-4050
pubmed:author
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
287-94
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1973
pubmed:articleTitle
Cell culture studies of a neonatal calf diarrhea virus.
pubmed:publicationType
Journal Article, Comparative Study