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rdf:type |
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lifeskim:mentions |
umls-concept:C0007382,
umls-concept:C0020616,
umls-concept:C0022013,
umls-concept:C0026239,
umls-concept:C0184633,
umls-concept:C0205102,
umls-concept:C0205198,
umls-concept:C0205474,
umls-concept:C0243144,
umls-concept:C0596901,
umls-concept:C1280500,
umls-concept:C1882726
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pubmed:issue |
1
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pubmed:dateCreated |
1973-3-8
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pubmed:abstractText |
1. The hypoglycaemic compound diphenyleneiodonium causes rapid and extensive swelling of rat liver mitochondria suspended in 150mm-NH(4)Cl, and in 150mm-KCl in the presence of 2,4-dinitrophenol and valinomycin. This indicates that diphenyleneiodonium catalyses a compulsory exchange of OH(-) for Cl(-) across the mitochondrial inner membrane. Br(-) and SCN(-) were the only other anions found whose exchange for OH(-) is catalysed by diphenyleneiodonium. 2. Diphenyleneiodonium inhibited state 3 respiration of mitochondria and slightly stimulated state 4 respiration with succinate or glutamate as substrate in a standard Cl(-)-containing medium. 3. Diphenyleneiodonium did not inhibit state 3 respiration significantly in two Cl(-)-free media (based on glycerol 2-phosphate or sucrose) but caused some stimulation of state 4. 4. In Cl(-)-containing medium diphenyleneiodonium only slightly inhibited the 2,4-dinitrophenol-stimulated adenosine triphosphatase and it had little effect in the absence of Cl(-). 5. The inhibition of respiration in the presence of Cl(-) is dependent on the Cl(-)-OH(-) exchange. 2,4-Dichlorodiphenyleneiodonium is ten times as active as diphenyleneiodonium both in causing swelling of mitochondria suspended in 150mm-NH(4)Cl and in inhibiting state 3 respiration in Cl(-)-containing medium. Indirect evidence suggests that the Cl(-)-OH(-) exchange impairs the rate of uptake of substrate anions. 6. It is proposed that stimulation of state 4 respiration in the absence of Cl(-) depends, at least in part, on an electrogenic uptake of diphenyleneiodonium cations. 7. Tripropyl-lead acetate, methylmercuric iodide and nine substituted diphenyleneiodonium derivatives also catalyse Cl(-)-OH(-) exchange across the mitochondrial membrane. 8. Diphenyleneiodonium is compared with the trialkyltin compounds, which are also known to mediate Cl(-)-OH(-) exchange and which have in addition strong oligomycin-like effects on respiration. It is concluded that diphenyleneiodonium is specific for catalysing anion-OH(-) exchange and will be a useful reagent for investigating membrane-dependent systems.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-13269376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-13313307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-13479399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-13560377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-13596366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-14228485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-14351681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-14424108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-16744823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-4131734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-4250571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-4250572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-4293928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-4378636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-4980514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-5099211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-5099217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-5126473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-5447428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-5469174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-5494221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-5502666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-5581324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-5649935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-5701680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-5701682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-5774477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4265024-5804494
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
129
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
39-54
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:4265024-Acetates,
pubmed-meshheading:4265024-Adenosine Triphosphatases,
pubmed-meshheading:4265024-Animals,
pubmed-meshheading:4265024-Bromides,
pubmed-meshheading:4265024-Catalysis,
pubmed-meshheading:4265024-Chlorides,
pubmed-meshheading:4265024-Iodine,
pubmed-meshheading:4265024-Ion Exchange,
pubmed-meshheading:4265024-Lead,
pubmed-meshheading:4265024-Membranes,
pubmed-meshheading:4265024-Mitochondria, Liver,
pubmed-meshheading:4265024-Organometallic Compounds,
pubmed-meshheading:4265024-Oxygen Consumption,
pubmed-meshheading:4265024-Quaternary Ammonium Compounds,
pubmed-meshheading:4265024-Rats
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pubmed:year |
1972
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pubmed:articleTitle |
Biochemical effects of the hypoglycaemic compound diphenyleneiodonnium. Catalysis of anion-hydroxyl ion exchange across the inner membrane of rat liver mitochondria and effects on oxygen uptake.
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pubmed:publicationType |
Journal Article,
In Vitro
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