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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1979-5-23
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pubmed:abstractText |
P1-Adenosine 5'-P2-o-nitrobenzyl pyrophosphate (nbzlppA) has been synthesized as a substrate for T4 RNA ligase catalyzed 3'-phosphorylation. Incubation of oligoribonucleotides and nbzlppA with RNA ligase yielded oligoribonucleotides having a 3'-o-(o-nitrobenzyl) phosphate. Photochemical removal of the o-nitrobenzyl group provided the free 3'-phosphate. Using [P2-32P] nbzlppA, 3'-termini of oligoribonucleotides could be labelled with 32P. This reaction was applied to modify the 3'-end of donor molecules in joining reaction with RNA ligase. A trinucleotide U-A-G was converted to U-A-Gpnbzl and phosphorylated with polynucleotide kinase. pU-A-Gpnbzl was then joined to an acceptor trinucleotide A-U-G to yield A-U-G-U-A-Gp.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/424301-1005114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/424301-10793670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/424301-1090929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/424301-17097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/424301-183186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/424301-200936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/424301-205838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/424301-274717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/424301-320212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/424301-4342972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/424301-4373468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/424301-4609429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/424301-4610584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/424301-4902612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/424301-598370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/424301-667012
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
443-54
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1979
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pubmed:articleTitle |
A new method for 3'-labelling of polyribonucleotides by phosphorylation with RNA ligase and its application to the 3'-modification for joining reactions.
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pubmed:publicationType |
Journal Article
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