| pubmed-article:4219284 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:4219284 | lifeskim:mentions | umls-concept:C0040300 | lld:lifeskim |
| pubmed-article:4219284 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:4219284 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:4219284 | lifeskim:mentions | umls-concept:C0003320 | lld:lifeskim |
| pubmed-article:4219284 | lifeskim:mentions | umls-concept:C0011740 | lld:lifeskim |
| pubmed-article:4219284 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:4219284 | lifeskim:mentions | umls-concept:C0439611 | lld:lifeskim |
| pubmed-article:4219284 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:4219284 | pubmed:dateCreated | 1975-11-5 | lld:pubmed |
| pubmed-article:4219284 | pubmed:abstractText | 1. A radioactive binding assay for Thy-1.1 alloantigen which functions in the presence of detergents was established by using glutaraldehyde-fixed thymocytes as target cells. Thy-1.1 activity in detergent extracts was then assayed by measuring inhibition of the binding assay. 2. Solubilization of Thy-1.1 from whole thymocytes, and their membranes by a large number of non-ionic detergents and deoxycholate was studied. In the same extracts Ag-B(4) histocompatibility antigenic activities were measured. With the exception of Nonidet P-40, the detergents did not affect the antigenicity of Thy-1.1, but only Lubrol-PX and deoxycholate gave effective solubilization as measured by activity remaining in the supernatant after centrifugation at 200000g for 40min. With Ag-B(4) antigen, Triton X-100, Triton X-67 and Nonidet P-40 gave effective solubilization as well as Lubrol-PX and deoxycholate. Solubilization of Thy-1.1 activity from leukaemia cells and a brain homogenate was also studied, but none of the non-ionic detergents gave satisfactory results with these tissues. 3. Extracts from thymocyte membranes were further examined by gel filtration and sucrose gradient centrifugation. The Thy-1.1 activity behaved as a single component in deoxycholate with a density similar to that of a globular protein, but in Lubrol-PX the antigen was contained in a low-density complex. In Lubrol-PX extracts Ag-B(4) was also found in aggregates not observed in deoxycholate. 4. The s(20,w) values for Thy-1.1 and Ag-B(4) antigens in deoxycholate were 2.4 and 4.4, and v values were 0.70 and 0.75 respectively. The Stokes radius observed for Thy-1.1 was 3.1nm and for Ag-B(4) 5.3nm. By using these values the molecular weights for the antigen-detergent complexes were calculated to be 28000 for Thy-1.1 and 100000 for Ag-B(4). | lld:pubmed |
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| pubmed-article:4219284 | pubmed:language | eng | lld:pubmed |
| pubmed-article:4219284 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:4219284 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:4219284 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:4219284 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:4219284 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:4219284 | pubmed:author | pubmed-author:WilliamsA FAF | lld:pubmed |
| pubmed-article:4219284 | pubmed:author | pubmed-author:ActionR TRT | lld:pubmed |
| pubmed-article:4219284 | pubmed:author | pubmed-author:MuirheadM LML | lld:pubmed |
| pubmed-article:4219284 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:4219284 | pubmed:volume | 143 | lld:pubmed |
| pubmed-article:4219284 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:4219284 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:4219284 | pubmed:pagination | 51-61 | lld:pubmed |
| pubmed-article:4219284 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:4219284 | pubmed:year | 1974 | lld:pubmed |
| pubmed-article:4219284 | pubmed:articleTitle | Preliminary characterization of Thy-1.1 and Ag-B antigens from rat tissues solubilized in detergents. | lld:pubmed |
| pubmed-article:4219284 | pubmed:publicationType | Journal Article | lld:pubmed |
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