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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1975-11-5
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pubmed:abstractText |
1. A procedure was devised which is suitable for the isolation of beta-lactamase I and beta-lactamase II from Bacillus cereus 569/H/9 on a large scale. After adsorption on to Celite both enzymes were eluted in good yield and separated by chromatography on Sephadex CM-50. 2. beta-Lactamase I was separated into three main components by isoelectric focusing and into two components by chromatography. 3. The Zn(2+)-requiring beta-lactamase II obtained by this procedure had a lower molecular weight (22000) than beta-lactamase I (28000) and also differed from the latter in containing one cysteine residue. 4. The beta-lactamase II contained no carbohydrate, but showed the thermostability of the enzyme isolated earlier as a protein-carbohydrate complex. 5. Amino acid analyses and tryptic-digest ;maps' indicate that some degree of homology between beta-lactamase I and beta-lactamase II is possible, but that beta-lactamase I is not composed of the entire sequence of beta-lactamase II together with an additional peptide fragment. 6. A 6-methylpenicillin and a 7-methylcephalosporin showed much lower affinities for both enzymes than did penicillins and cephalosporins themselves.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-13303986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-13319283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-13513014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-13882319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-14072511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-14404782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-14484808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-15395390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-16748273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-4570347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-4956798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-4967769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-4983912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-4986078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-4990588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-4990590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-4996025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-4999161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-5003529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-5126472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-5334062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-5435685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-5472175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-5806584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-5862414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4219278-6069166
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
143
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
115-27
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:4219278-Amino Acids,
pubmed-meshheading:4219278-Bacillus cereus,
pubmed-meshheading:4219278-Bacteriological Techniques,
pubmed-meshheading:4219278-Carbohydrates,
pubmed-meshheading:4219278-Cephalosporins,
pubmed-meshheading:4219278-Culture Media,
pubmed-meshheading:4219278-Hydrogen-Ion Concentration,
pubmed-meshheading:4219278-Hydrolysis,
pubmed-meshheading:4219278-Molecular Weight,
pubmed-meshheading:4219278-Penicillin G,
pubmed-meshheading:4219278-Penicillinase,
pubmed-meshheading:4219278-Temperature,
pubmed-meshheading:4219278-Ultrafiltration
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pubmed:year |
1974
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pubmed:articleTitle |
Separation, purification and properties of beta-lactamase I and beta-lactamase II from Bacillus cereus 569/H/9.
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pubmed:publicationType |
Journal Article,
In Vitro
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