Linked Life Data

42173

Source:http://linkedlifedata.com/resource/pubmed/id/42173

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1980-2-15
pubmed:abstractText
Only 35-50% of the label accumulated after incubation of cultured Sertoli cells with 3H-testosterone was readily extractable with 0.4 M KCl during a 1 h exposure. The degree of extractability was relatively constant over the pH range 7.0-8.5 but could be increased by prolonged (15 h) exposure. While 0.1 M KCl extracted a measurable amount of label, 0.4 M KCl was significantly more efficient. Furthermore, a higher proportion of the material extracted with 0.4 M KCl was associated with macromolecular species. After a 45 min exposure to 3H-testosterone, the nuclear fraction contained primarily labeled testosterone and its 5 alpha-reduced metabolites. The relative distribution of these metabolites between salt-resistant and readily extractable forms varied between experiments. In contrast, 3H-R1881 (17 beta-hydroxy-17-methylestra-4,9,11-trien-3-one) remained essentially intact in the nuclear fraction but also was only 35% extractable with 0.4 M KCl. In conclusion, although the quantitative aspects of salt extractability appear to depend to some extent upon the extraction conditions, it is apparent that the Sertoli cell nuclear fraction accumulates a significant amount of androgen in a form which is relatively resistant to removal with 0.4 M KCl. The biological significance of this phenomenon remains to be established.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0039-128X
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
401-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
Effect of ionic strength on the interaction of androgens with Sertoli cell nuclei.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.