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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1974-10-24
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pubmed:abstractText |
The Streptococcus mutans group d antigen of strain B13 has been purified and characterized with respect to chemical composition and immunochemical properties. The antigen was extracted from lyophilized cells or cell walls by using 5% trichloroacetic acid at 5 C for 16 h. The antigen could also be extracted with water or 0.01 N HCl at 100 C for 20 min. The antigen was purified by ion-exchange and gel chromatography and was found to contain 96% carbohydrate, 1.6% protein, and 0.3% phosphorus. Characterization by gas chromatography indicated that the polysaccharide was composed of galactose and glucose in a 2:1 ratio. The antigen contained two serologically active sites: one site specific for group d and a second site common to both group d and group a strains. Agar diffusion and immunoelectrophoresis indicated that the two sites existed on a single molecule. The immunological specificity of the group d polysaccharide site depended on a terminal d-galactose. The purified B13 antigen did not react with antisera specific for the glycerol teichoic acid from streptococci. Anti-d serum rapidly agglutinated whole cells, indicating that the antibody receptor sites of the polysaccharide antigen were at the surface of the streptococcal cell.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-13578996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-13641241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-13700062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-13823312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-13831587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-14378162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-16557984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-16562065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-4128403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-4174224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-4199154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-4202892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-4205950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-4556646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-4564674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-4575463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-4582634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-4629249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-4735375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-4992207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-5111564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-5226774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-5237347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-5551636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4212286-6056776
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alcohol Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Carbohydrates,
http://linkedlifedata.com/resource/pubmed/chemical/Cellulase,
http://linkedlifedata.com/resource/pubmed/chemical/Dextranase,
http://linkedlifedata.com/resource/pubmed/chemical/Galactose,
http://linkedlifedata.com/resource/pubmed/chemical/Galactosidases,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose Oxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Glucosidases,
http://linkedlifedata.com/resource/pubmed/chemical/Immune Sera,
http://linkedlifedata.com/resource/pubmed/chemical/Muramidase,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphorus,
http://linkedlifedata.com/resource/pubmed/chemical/Polysaccharides, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Teichoic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
361-8
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:4212286-Adsorption,
pubmed-meshheading:4212286-Alcohol Oxidoreductases,
pubmed-meshheading:4212286-Animals,
pubmed-meshheading:4212286-Antibody Specificity,
pubmed-meshheading:4212286-Antigens, Bacterial,
pubmed-meshheading:4212286-Carbohydrates,
pubmed-meshheading:4212286-Cell Wall,
pubmed-meshheading:4212286-Cellulase,
pubmed-meshheading:4212286-Chromatography, Gas,
pubmed-meshheading:4212286-Dextranase,
pubmed-meshheading:4212286-Galactose,
pubmed-meshheading:4212286-Galactosidases,
pubmed-meshheading:4212286-Glucose Oxidase,
pubmed-meshheading:4212286-Glucosidases,
pubmed-meshheading:4212286-Immune Sera,
pubmed-meshheading:4212286-Immunodiffusion,
pubmed-meshheading:4212286-Immunoelectrophoresis,
pubmed-meshheading:4212286-Muramidase,
pubmed-meshheading:4212286-Phosphorus,
pubmed-meshheading:4212286-Polysaccharides, Bacterial,
pubmed-meshheading:4212286-Precipitin Tests,
pubmed-meshheading:4212286-Proteins,
pubmed-meshheading:4212286-Rabbits,
pubmed-meshheading:4212286-Streptococcus,
pubmed-meshheading:4212286-Teichoic Acids,
pubmed-meshheading:4212286-Trypsin
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pubmed:year |
1974
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pubmed:articleTitle |
Purification and characterization of Streptococcus mutans group d cell wall polysaccharide antigen.
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pubmed:publicationType |
Journal Article
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