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rdf:type |
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lifeskim:mentions |
umls-concept:C0003241,
umls-concept:C0004561,
umls-concept:C0024264,
umls-concept:C0039194,
umls-concept:C0086418,
umls-concept:C0205263,
umls-concept:C0220781,
umls-concept:C0391871,
umls-concept:C1514485,
umls-concept:C1521761,
umls-concept:C1533691,
umls-concept:C1704675,
umls-concept:C1749467,
umls-concept:C1883254
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pubmed:issue |
5
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pubmed:dateCreated |
1973-12-15
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pubmed:abstractText |
Relatively pure populations of human T and B lymphocytes were obtained from blood and tonsils using density gradient centrifugation in bovine serum albumin. Antigen alone was incapable of triggering the B lymphocyte into blast transformation or to secrete antibody. However, supernatants from tetanus toxoid-stimulated T cells obtained from immune donors contained a factor mitogenic for B lymphocytes. 50-60% of B cells responded to this lymphocyte mitogenic factor (LMF) by proliferation, loss of C3 reactivity, and change to a secretory state. LMF-stimulated B cells exhibited a three- to fivefold increase in protein secretion and a six- to eightfold increase in gamma G globulin secretion. De novo secreted IgG had specificity directed to the tetanus toxoid present in the LMF containing T-cell supernatants. This was confirmed by an increase in the number of indirect plaque-forming cells to tetanus toxoid-coated sheep red blood cells after stimulation of B cells with LMF. It is proposed that in the course of the response to a previously encountered protein antigen, sensitized human T cells emit a signal in the form of a soluble product that, together with antigen, triggers B cells into division and antibody secretion. The experimental model utilized can be adapted to study human T-B cell cooperation under various conditions in normal individuals and in individuals with immunodeficiency diseases.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-13673056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-14029139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-14240539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-4101362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-4109111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-4113234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-4115708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-4120290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-4179930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-4192568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-4301109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-4503682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-4538839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-4578158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-4945475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-4945477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-4986654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-5063805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-5064225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-5066511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-5099924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-5147490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-5542945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-5691986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-5806584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-6034749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4200776-6061717
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1007
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
138
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1230-47
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pubmed:dateRevised |
2010-6-22
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pubmed:meshHeading |
pubmed-meshheading:4200776-Animals,
pubmed-meshheading:4200776-Antibody Formation,
pubmed-meshheading:4200776-Antibody-Producing Cells,
pubmed-meshheading:4200776-Antigen-Antibody Reactions,
pubmed-meshheading:4200776-Autoradiography,
pubmed-meshheading:4200776-B-Lymphocytes,
pubmed-meshheading:4200776-Blood Proteins,
pubmed-meshheading:4200776-Cell Division,
pubmed-meshheading:4200776-Cells, Cultured,
pubmed-meshheading:4200776-DNA,
pubmed-meshheading:4200776-Erythrocytes,
pubmed-meshheading:4200776-Humans,
pubmed-meshheading:4200776-Immunodiffusion,
pubmed-meshheading:4200776-Immunoelectrophoresis,
pubmed-meshheading:4200776-Mitogens,
pubmed-meshheading:4200776-Palatine Tonsil,
pubmed-meshheading:4200776-Serum Albumin, Bovine,
pubmed-meshheading:4200776-Sheep,
pubmed-meshheading:4200776-T-Lymphocytes
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pubmed:year |
1973
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pubmed:articleTitle |
Interaction of human thymus-derived and non-thymus-derived lymphocytes in vitro. Induction of proliferation and antibody synthesis in B lymphocytes by a soluble factor released from antigen-stimulated T lymphocytes.
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pubmed:publicationType |
Journal Article
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