Linked Life Data

412251

Source:http://linkedlifedata.com/resource/pubmed/id/412251

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4321
pubmed:dateCreated
1978-1-27
pubmed:databankReference
pubmed:abstractText
A gene for somatostatin, a mammalian peptide (14 amino acid residues) hormone, was synthesized by chemical methods. This gene was fused to the Escherichia coli beta-galactosidase gene on the plasmid pBR322. Transformation of E. coli with the chimeric plasmid DNA led to the synthesis of a polypeptide including the sequence of amino acids corresponding to somatostatin. In vitro, active somatostatin was specifically cleaved from the large chimeric protein by treatment with cyanogen bromide. This represents the first synthesis of a functional polypeptide product from a gene of chemically synthesized origin.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0036-8075
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
198
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1056-63
pubmed:dateRevised
2007-3-19
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin.
pubmed:publicationType
Journal Article