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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1973-1-18
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pubmed:abstractText |
A new thermosensitive mutant of Escherichia coli deficient in cell division was isolated by means of membrane filtration after nitrosoguanidine mutagenesis. The mutant cells grow normally at 30 C but stop dividing immediately after shift to 42 C, resulting in multinucleated filaments lacking septa. The number of colony-forming units does not decrease for at least 6 hr at 42 C. The maximum length of the filaments is 10 to 16 times that of normal cells. Addition of a high concentration of NaCl fails to stimulate cell division at 42 C. The filaments formed at 42 C divide abruptly 30 min after shift to 30 C, and synchronous increase of cell number is shown for 3 hr. The macromolecular synthesis of protein and nucleic acids at 42 C is normal on the whole. The cell division shown after the shift from 42 to 30 C is observed in the absence of thymine, but not in the presence of chloramphenicol or in a medium deficient in amino acids. However, the filament can divide to some extent in the presence of chloramphenicol if some protein synthesis is allowed to proceed at 30 C before the addition of the antibiotic. The elongated cells divide at 42 C provided that they are exposed to 30 C before being shifted to high temperature.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-13319263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-13764136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-13975866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-14237063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-16559077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-4870278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-4879557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-4886279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-4892005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-4892011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-4905540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-4905995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-4908787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-4919490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-4922211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-4929866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-4939776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-4964484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-5325383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4117582-5543950
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenine,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Isotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Leucine,
http://linkedlifedata.com/resource/pubmed/chemical/Mutagens,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrosoguanidines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Bacterial
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
112
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
959-66
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:4117582-Adenine,
pubmed-meshheading:4117582-Bacterial Proteins,
pubmed-meshheading:4117582-Carbon Isotopes,
pubmed-meshheading:4117582-Cell Division,
pubmed-meshheading:4117582-Chloramphenicol,
pubmed-meshheading:4117582-Culture Media,
pubmed-meshheading:4117582-DNA, Bacterial,
pubmed-meshheading:4117582-Escherichia coli,
pubmed-meshheading:4117582-Filtration,
pubmed-meshheading:4117582-Leucine,
pubmed-meshheading:4117582-Microscopy, Electron,
pubmed-meshheading:4117582-Microscopy, Phase-Contrast,
pubmed-meshheading:4117582-Models, Biological,
pubmed-meshheading:4117582-Mutagens,
pubmed-meshheading:4117582-Mutation,
pubmed-meshheading:4117582-Nitrosoguanidines,
pubmed-meshheading:4117582-RNA, Bacterial,
pubmed-meshheading:4117582-Staining and Labeling,
pubmed-meshheading:4117582-Temperature
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pubmed:year |
1972
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pubmed:articleTitle |
Mutant of Escherichia coli with thermosensitive protein in the process of cellular division.
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pubmed:publicationType |
Journal Article
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