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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1986-4-8
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pubmed:abstractText |
Physicochemical properties of the soluble Mn2+-dependent adenylate cyclase (MnAC) in the blue fox testis were examined. MnAC activity in the cytosol fraction (106,000 g) migrated in a linear sucrose gradient as a single peak with a sedimentation coefficient (S20,W) of 4.2 Gel filtration on a Sephadex G-200 column revealed an Einstein-Stokes radius of 28 A and a frictional ratio of 1.15, indicating a molecular weight of approximately 51,000. The MnAC migrated as a single peak of activity during polyacrylamide gel electrophoresis with an Rf of 0.52. The isoelectric point (pI) estimated by isoelectric focusing was 5.5.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0148-5016
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
53-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:4096576-Adenylate Cyclase,
pubmed-meshheading:4096576-Animals,
pubmed-meshheading:4096576-Centrifugation, Density Gradient,
pubmed-meshheading:4096576-Cytosol,
pubmed-meshheading:4096576-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:4096576-Foxes,
pubmed-meshheading:4096576-Isoelectric Focusing,
pubmed-meshheading:4096576-Male,
pubmed-meshheading:4096576-Manganese,
pubmed-meshheading:4096576-Molecular Weight,
pubmed-meshheading:4096576-Testis
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pubmed:year |
1985
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pubmed:articleTitle |
Physicochemical properties of the soluble Mn2+-dependent adenylate cyclase in the blue fox testis.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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