Linked Life Data

4092051

Source:http://linkedlifedata.com/resource/pubmed/id/4092051

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
27
pubmed:dateCreated
1986-4-7
pubmed:abstractText
Alkyldihydroxyacetonephosphate synthase (alkyl-DHAP synthase) catalyzes the exchange of the ester-linked fatty acid of 1-O-acyldihydroxyacetone phosphate (1-O-acyl-DHAP) for a fatty alcohol that is attached in an ether linkage to form 1-O-alkyldihydroxyacetone phosphate (1-O-alkyl-DHAP). In our continuing investigation of the mechanism of this enzyme, we have examined the fatty acid released during the reaction. In contrast to the reports of others using whole microsomes, we found that the cleavage of fatty acid by purified preparations of alkyl-DHAP synthase was dependent on the presence of the cosubstrate, fatty alcohol. Furthermore, the amount of fatty acid produced was equivalent to the alkyl-DHAP formed. Our previously proposed detailed mechanism for alkyl-DHAP synthase predicted that the fatty acid should retain both of the carboxyl ester oxygens upon cleavage. Reactions carried out with palmitoyl-[18O]DHAP as substrate yielded [18O]palmitic acid as the product in agreement with this scheme.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8012-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Alkyldihydroxyacetonephosphate synthase mechanism: 18O studies of fatty acid release from acyldihydroxyacetone phosphate.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't