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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1986-3-26
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pubmed:abstractText |
The concept that creatine phosphokinase is bound to the outer surface of the heart mitochondrial inner membrane originated from observations that the enzyme is retained by water-swollen heart mitochondria and by digitonin-treated heart mitochondria suspended in isotonic sucrose. The present study establishes that digitonin-treated mitochondria release creatine phosphokinase in isotonic KCl, and other investigators have reported an identical response for the water-swollen organelles. These observations suggest that mitochondrial creatine phosphokinase is not bound to the outer surface of the inner membrane at a site adjacent to the adenine nucleotide translocase under physiologic conditions.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0145-479X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
295-303
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:4086487-Adenylate Kinase,
pubmed-meshheading:4086487-Animals,
pubmed-meshheading:4086487-Binding Sites,
pubmed-meshheading:4086487-Buffers,
pubmed-meshheading:4086487-Cattle,
pubmed-meshheading:4086487-Creatine Kinase,
pubmed-meshheading:4086487-Digitonin,
pubmed-meshheading:4086487-Hydroxymercuribenzoates,
pubmed-meshheading:4086487-Intracellular Membranes,
pubmed-meshheading:4086487-Mitochondria, Heart,
pubmed-meshheading:4086487-Osmolar Concentration,
pubmed-meshheading:4086487-Potassium Chloride,
pubmed-meshheading:4086487-Sulfhydryl Reagents
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pubmed:year |
1985
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pubmed:articleTitle |
Effects of ionic strength and sulfhydryl reagents on the binding of creatine phosphokinase to heart mitochondrial inner membranes.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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