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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1986-2-19
|
| pubmed:abstractText |
A simple reliable and specific binding assay for the estimation of plasma (or serum) aldosterone-binding globulins (ABGs) is described. The method is based on the determination of the aldosterone-binding capacity of diluted plasma (with water 1:5), and relating it to the total aldosterone concentration in the same sample. The method distinguishes between the heat-labile and heat-stable ABG, the binding to the latter being determined following heating of plasma at 60 degrees C for 25 min. The binding to the heat-labile protein is determined by subtraction of the value for the binding to the heat-stable protein from the binding of the nonheated diluted plasma. Free aldosterone is separated from the bound fraction by adsorption of the former to dextran-coated charcoal. Two different concentrations of [3H]-aldosterone are used throughout the assay. Data obtained by incorporating chromatographic separation into the cross-reactivity procedure using several steroids are presented as evidence for the specificity of the method. This chromatography separates plasma ABG from corticosteroid-binding globulin. In 316 male or female controls, ABG capacity was 9.7 +/- 0.38% (SE)-range 2-17. Samples in females were taken during the first 8 days from the onset of menstruation. Higher ABG-binding capacity (p less than 0.001) was found during pregnancy.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0252-1164
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
3
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
314-22
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:4075695-Adolescent,
pubmed-meshheading:4075695-Adult,
pubmed-meshheading:4075695-Aged,
pubmed-meshheading:4075695-Aldosterone,
pubmed-meshheading:4075695-Binding, Competitive,
pubmed-meshheading:4075695-Carrier Proteins,
pubmed-meshheading:4075695-Child,
pubmed-meshheading:4075695-Child, Preschool,
pubmed-meshheading:4075695-Female,
pubmed-meshheading:4075695-Hot Temperature,
pubmed-meshheading:4075695-Humans,
pubmed-meshheading:4075695-Male,
pubmed-meshheading:4075695-Middle Aged,
pubmed-meshheading:4075695-Pregnancy,
pubmed-meshheading:4075695-Radioligand Assay,
pubmed-meshheading:4075695-Reference Values,
pubmed-meshheading:4075695-Serum Globulins,
pubmed-meshheading:4075695-Sex Factors,
pubmed-meshheading:4075695-Transcortin
|
| pubmed:year |
1985
|
| pubmed:articleTitle |
Simple reproducible competitive radioligand assay for plasma protein binding of aldosterone.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|