4074841

Source:http://linkedlifedata.com/resource/pubmed/id/4074841

Download in:

Switch to

Custom View

Named Graph Language Inference

Statements in which the resource exists as a subject.
Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0001511, umls-concept:C0014792, umls-concept:C0017596, umls-concept:C0596545, umls-concept:C0683283, umls-concept:C1521991
pubmed:issue	5
pubmed:dateCreated	1986-2-18
pubmed:abstractText	We have studied the detachment of unfixed red cells from glass coverslips under unit gravity and by centrifugation in buffered isotonic solutions over a range of ionic strengths. Cell-glass contact areas and separation distances were measured by quantitative interference reflection microscopy. Detachment under unit gravity is highly dependent on ionic strength: dilution increases electrostatic repulsion and greatly reduces the proportion of adherent cells. However, even at 1.5 mM some cells stick. Over the range 3-110 mM such adherent cells are progressively removed by increasing centrifugal forces, but in a manner virtually independent of ionic strength. This fact, together with the irreversibility of pre-adherent cells as ionic strength is progressively reduced, as well as the resistance of cells to lateral shearing forces, provide evidence sufficient to reject the notion of secondary minimum adhesion for unfixed cells at any ionic strength down to 1.5 mM. We conclude that all unfixed cells that stick at ionic strengths from 157 to 1.5 mM make molecular contacts with glass. Comparison with long range force calculations suggests that to penetrate the electrostatic repulsion barrier the contact regions are unlikely to have average surface properties. A new method that compares frequency distributions of contact areas with responses to detachment forces shows that detachment forces are not linearly related to contact areas. This lack of relationship is less clearly evident for rigid glutaraldehyde-fixed cells and may therefore depend on the degree of cellular deformability.
pubmed:commentsCorrections	http://linkedlifedata.com/resource/pubmed/commentcorrection/4074841-14233155, http://linkedlifedata.com/resource/pubmed/commentcorrection/4074841-239006, http://linkedlifedata.com/resource/pubmed/commentcorrection/4074841-262429, http://linkedlifedata.com/resource/pubmed/commentcorrection/4074841-4364148, http://linkedlifedata.com/resource/pubmed/commentcorrection/4074841-4925905, http://linkedlifedata.com/resource/pubmed/commentcorrection/4074841-5639051, http://linkedlifedata.com/resource/pubmed/commentcorrection/4074841-6259246, http://linkedlifedata.com/resource/pubmed/commentcorrection/4074841-6630305, http://linkedlifedata.com/resource/pubmed/commentcorrection/4074841-6630306, http://linkedlifedata.com/resource/pubmed/commentcorrection/4074841-6640058, http://linkedlifedata.com/resource/pubmed/commentcorrection/4074841-7364878, http://linkedlifedata.com/resource/pubmed/commentcorrection/4074841-7364879, http://linkedlifedata.com/resource/pubmed/commentcorrection/4074841-822894
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/0370626
pubmed:citationSubset	IM
pubmed:status	MEDLINE
pubmed:month	Nov
pubmed:issn	0006-3495
pubmed:author	pubmed-author:GingellDD, pubmed-author:TrommlerAA, pubmed-author:WolfHH
pubmed:issnType	Print
pubmed:volume	48
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	835-41
pubmed:dateRevised	2009-11-18
pubmed:meshHeading	pubmed-meshheading:4074841-Cell Adhesion, pubmed-meshheading:4074841-Electrophysiology, pubmed-meshheading:4074841-Erythrocytes, pubmed-meshheading:4074841-Glass, pubmed-meshheading:4074841-Humans, pubmed-meshheading:4074841-Male, pubmed-meshheading:4074841-Microscopy, Interference
pubmed:year	1985
pubmed:articleTitle	Red blood cells experience electrostatic repulsion but make molecular adhesions with glass.
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't