Linked Life Data

4065225

Source:http://linkedlifedata.com/resource/pubmed/id/4065225

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1986-1-6
pubmed:abstractText
The influence of cell shape on phenotypic expression was studied in chick vertebral chondroblasts maintained for several weeks in suspension culture. To monitor phenotypic expression, synthesis of proteoglycans was studied in cultures of freshly-isolated 1-day-old chondroblasts and 1-to-6-week-old chondroblasts. The rate of proteoglycan synthesis was virtually identical in 1-week or older chondroblasts; however, this rate was 3- to 5-fold higher than in 1-day-old cells. When compared to the latter cells, the various populations of older chondroblasts synthesized monomers of the major cartilage proteoglycan (KS: CS-PG) of slightly lower molecular size and a lower level of unsubstituted N-acetylgalactosamine residues on their core protein but with similar chondroitin sulfate chains and levels of O-linked oligosaccharides. At no time of culture were changes in the proportions of the major vs the minor cartilage proteoglycans detected. The results suggest that in contrast to epithelioid chondroblasts in standard monolayer cultures studied previously, the round floating chondroblasts express very stable biosynthetic properties for a prolonged time in suspension. The distinct biosynthetic properties of 1-day-old chondroblasts are discussed in terms of an initial, transitory response to the culture condition and in relation to regulatory mechanisms for proteoglycan elaboration.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0014-4827
pubmed:author
pubmed:issnType
Print
pubmed:volume
161
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
381-92
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Stable phenotypic expression by chick chondroblasts in long-term suspension cultures as determined by proteoglycan analysis.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.