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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1985-12-4
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pubmed:abstractText |
The proteins from labelled human spleen membranes and polymorphonuclear leucocytes which bind to the iC3b fragment of complement component C3 were prepared by iC3b-Sepharose chromatography in the presence of bivalent cations. Complement receptor type 3(CR3) was eluted from iC3b-Sepharose by removal of bivalent cations. Complement receptors type 1 and 2 (present in spleen but not in polymorphonuclear leucocytes) were sequentially eluted by an NaCl gradient. An additional protein of Mr 135 000 was eluted from iC3b-Sepharose under the same conditions as those used to elute CR3. Preabsorption of the starting material on an anti-(CR3 beta-subunit) antibody column before iC3b-Sepharose chromatography removed the alpha- and beta-chains of CR3 and the 135 000-Mr protein. Preabsorption with iC3b-Sepharose before the anti-(CR3 beta-subunit) antibody column showed that iC3b binds CR3 and p150,95, the smallest member of the group of three homologous proteins that share the same beta-subunit.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-2580022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-268620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-3156185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-3871945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-6163824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-6175907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-6196430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-6224880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-6225125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-6225820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-6239618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-6242465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-6339253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-6356429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-6601277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-6607952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-6746906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-6967510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-6976275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-7153706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-7360115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-833545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4062888-89034
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
231
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
233-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:4062888-Antibodies, Monoclonal,
pubmed-meshheading:4062888-Cell Membrane,
pubmed-meshheading:4062888-Chromatography, Affinity,
pubmed-meshheading:4062888-Complement C3b,
pubmed-meshheading:4062888-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:4062888-Humans,
pubmed-meshheading:4062888-Neutrophils,
pubmed-meshheading:4062888-Spleen
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pubmed:year |
1985
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pubmed:articleTitle |
Isolation of complement-fragment-iC3b-binding proteins by affinity chromatography. The identification of p150,95 as an iC3b-binding protein.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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