Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5 Pt 1
pubmed:dateCreated
1985-12-13
pubmed:abstractText
We have isolated a nucleus-basal body complex from Chlamydomonas reinhardtii. The complex is strongly immunoreactive to an antibody generated against a major protein constituent of isolated Tetraselmis striata flagellar roots (Salisbury, J. L., A. Baron, B. Surek, and M. Melkonian, J. Cell Biol., 99:962-970). Electrophoretic and immunoelectrophoretic analysis indicates that, like the Tetraselmis protein, the Chlamydomonas antigen consists of two acidic isoforms of approximately 20 kD. Indirect immunofluorescent staining of nucleus-basal body complexes reveals two major fibers in the connector region, one between each basal body and the nucleus. The nucleus is also strongly immunoreactive, with staining radiating around much of the nucleus from a region of greatest concentration at the connector pole. Calcium treatment causes shortening of the connector fibers and also movement of nuclear DNA towards the connector pole. Electron microscopic observation of negatively stained nucleus-basal body complexes reveals a cluster of approximately 6-nm filaments, suspected to represent the connector, between the basal bodies and nuclei. A mutant with a variable number of flagella, vfl-2-220, is defective with respect to the nucleus-basal body association. This observation encourages us to speculate that the nucleus-basal body union is important for accurate basal body localization within the cell and/or for accurate segregation of parental and daughter basal bodies at cell division. A physical association between nuclei and basal bodies or centrioles has been observed in a variety of algal, protozoan, and metazoan cells, although the nature of the association, in terms of both structure and function, has been obscure. We believe it likely that fibrous connectors homologous to those described here for Chlamydomonas are general features of centriole-bearing eucaryotic cells.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-1098148, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-1158970, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-1273306, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-13139273, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-149796, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-236308, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-3972905, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-4157379, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-4424205, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-455435, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-4615103, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-4682900, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-528590, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-5341020, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-5664210, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-571342, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-6370329, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-6381510, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-6714241, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-6725407, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-6734733, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-6802199, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-6853600, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-6991018, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-7056798, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-7107728, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-7130271, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-7151168, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-7328123, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-7348602, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-805149, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-923388, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-925090, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055898-927525
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
101
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1903-12
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
A nucleus-basal body connector in Chlamydomonas reinhardtii that may function in basal body localization or segregation.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't